Identification of Arsenite-resistance in Trypanosomes by Isozyme Characterization

錐蟲對砷化物抗藥性的異構酶分析

張琪(Chi Chang) ； 江亭誼(Ting-Yi Chiang) ； 黃尹虹(Yin-Hung Huang) ； 趙大衛(David Chao)

《師大生物學報》 33卷2期 (1998/12) Pp. 85-93

https://doi.org/10.29812/BBNTNU.199812.0002

摘要

治療錐蟲病的主要困難之一就是錐蟲的抗藥性。如果能夠儘早辨認出錐蟲的抗藥株，即可使用不同藥物的組合而達到最好的治療效果。治療錐蟲病最主要的藥為砷化物。本研究在探討錐蟲缺乏葡萄糖-6-磷酸去氫酶與其對砷化物抗藥性的關係。實驗所用的錐蟲培養在27℃的培養液中，包括Tulahuen（TL）株及Corpus Christi（CC）株的枯西氏錐蟲（Trypanosoma cruzi）、自台北紅帶錐鼻蟲分離出的錐蟲（Trypanosoma conorhini）、及自墾丁分離出的蝙蝠錐蟲。將錐蟲培養在不同濃度的砷化鈉中，每隔6至12小時的間隔計算存活錐蟲的數目。結果蝙蝠錐蟲在低濃度下即迅速死亡，對砷最為敏感；而CC株枯西氏錐蟲則因其在高濃度的砷化鈉培養液中仍可維持長時間的生存，為對砷抗藥性的蟲株。比較葡萄糖-6-磷酸去氫酶及磷酸葡萄糖異構酶的電泳圖譜，顯示不同蟲株的枯西氏錐蟲具有多樣性的異構酶，並且TL蟲株的異構酶電泳圖譜都比CC蟲株多二條色帶。紅帶錐鼻蟲錐蟲及蝙蝠錐蟲具有相似的磷酸葡萄糖異構酶，且只有枯西氏錐蟲CC株沒有任何葡萄糖-6-磷酸去氫酶的異構酶。錐蟲抗砷性與葡萄糖-6-磷酸去氫酶的缺乏之間的關係值得探討，而葡萄糖-6-磷酸去氫酶的缺乏可作為錐蟲抗砷性確認的簡易指標之一。

關鍵字

錐蟲 ； 抗砷性 ； 葡萄糖-6-磷酸去氫酶

並列摘要

Drug-resistance is one of the major problems ill the treatment of trypanosomiasis. If drug resistant str.uus could be identified car lice different combination of drugs could be used for better results. Arsenite containing drugs are still the most widely used drugs so far. This study intended to correlate arsenite-resistance with isozyme profiles in trypanosomes Tulahuen (TL) and Corpus Christi (CC) strains of T. cruzi. Trypanosoma conorhini isolated form Triatoma rubrofasciata in Taipei, as well as a chiropteran trypanosome isolated from Kenting were cultivated at 27°C and used for comparisons, Their survivals in different concentrations of sodium arsenite were monitored every 6-12 hours. The bat-trypanosomes were identified as arsenite-sensitive species since they died at low concentration while CC strain of T. cruzi was considered as an arsenite-resistanst strain as the trypanosomes survived even at a very high concentration. The electrophoretic morbilities of glucose-6-phosphatc dehydrogenase (G6PD) and phosphoglucose isomerase (PGI) isozymes were also compared. The results revealed as isozyme polymorphism in different strains T. cruzi and TL strain produced more than one isozyme bands in both isozyme cases. Similar PGI isozyme pattern was observed in T. conorhini and the bat-trypanosome, Uuiquely, there was not any isozyme bands of G6PD displayed by CC strain or T. cruzi. The correlation between arsenite-resistance and G6PD deficiency required more investigation and G6PD deficiency could be considered as all easier method for the identification of arsenite-resistance in trypanosomes.

並列關鍵字

Trypanosome ； Arsenite-resistance ； Glucose-6-phosphatc dehydrogenase