The objective of this research was to evaluate the effects of localized organic medium (dairy waste, rice hull, bagasse, and winter mushroom waste) on the nutrient contents of papaya seedlings. The results indicated that the nutrient contents of papaya seedlings were significantly affected by the nutrient characteristics of organic medium. The nutrient uptakes of papaya seedlings were lowered on the treatments of growth medium applied with sand or peat moss. Therefore, the most efficient way to develope localized organic medium is to control their element contents under safety requirements and understand the availability of nutrient of medium.