高粱子實單寧含量分析法之比較及差異與分佈之探討

Evaluation of Three Methods to Determine Grain Tannins Contents in Sorghum and Studies on Tannin Contents Difference among Panicle and Distribution in Sorghum Grain

張隆仁(Long-Zen Chang) ； 李台強(Tai-Chyang Lee)

《臺中區農業改良場研究彙報》 51期 (1996/06) Pp. 21-29

https://doi.org/10.6956/BTDAIS.199606.0021

摘要

本試驗以包括雜交高粱台中五號之親本2R、80B及4個具不同子實單寧含量之姊妹系（sister line）即TUI-1、TUI-2、TUI-3和TUI-4等六個高粱自交系為材料，分別以香草醛鹽酸分析法（V-HCl）、香草醛鹽酸修正法（MV-HCl）和香草醛鹽酸24 hr萃取之修正法（MV-HCl，24-hr extraction）等三種高粱子實單寧含量分析法測其單寧含量進行比較。參試品系以三種分析法分析結果顯示，分析法間有極顯著的差異存在，參試品系間則有顯著差異。三種分析法中以MV-HCl（24-hr）之分析法最易判別出品系間子實單寧含量之差異性，依單寧含量之高低，可將6品系區別為TUI-4、TUI-3、TUI-2、TUI-1及（80B、2R）等5群；其次為MV-HCl分析法可區別為TUI-4、TUI-3、（TUI-2、TUI-1）及（80B、2R）等4群；而V-HCl分析法則僅能將其區別為TUI-4、TUI-3及（TUI-2、TUI-1、80B、2R）等三群。不同穗部位之子實經取樣分析結果其單寧含量不具顯著性之差異。另子實內單寧含量分佈情形之分析結果指出，存在部位之含量百分比依不同品種而異，以種皮層及果皮部含有較高的單寧含量。

關鍵字

高粱 ； 單寧 ； 分析法

並列摘要

Six inbred lines of sorghum that named 2R, 80B, TUI-1, TUI-2, TUI-3 and TUI-4 were analyzed by three assays: vanillin hydrochloric acid (V-HCl), modified vanillin hydrochloric acid (MV-HCl) and MV-HCl (24-hr extraction) for grain tannin contents. The result data showed that there were significant differences in tannin content among three methods, and highly significant difference among six inbred lines. The method of MV-HCl (24-hr) showed the most sensitive to detecting levels of tannin among these lines. By this method, the detected values of tannin contents for six inbred lines from high to low, can be grouped into five groups, that were TUI-4, TUI-3, TUI-2, TUI-1 and (80B, 2R) ; And by MV-HCl method ,its' can be grouped into four groups, that were TUI-4, TUI-3, (TUI-2, TUI-1) and (80B, 2R); While the final method of V-HCl only can grouped six lines into three groups, that were THI-4, TUI-3, (TUI-2, TUI-1, 80B, 2R). The grain samples that derived from upper, middle and lower part of sorghum panicle, there were no significant differences in tannin contents among them. Further experiment revealed that 64%, 88% (TUI-2) and 99% (TUI-3 and TUI-4) of tannins were distributed in the pericarp and testa of sorghum grain. Endosperm and germ parts contained only small amount of it.

並列關鍵字

sorghum (Sorghum bicolor (L.) Moench) ； tannins ； analyzed method