兩種速測法在果樹葉片葉綠素含量測定之應用

Two Rapid Determination Methods for Total Chlorophyll Contentin Fruit Tree Leaves

張致盛(Chih-Sheng Chang) ； 張林仁(Lin-Ren Chang)

《臺中區農業改良場研究彙報》 59期 (1998/06) Pp. 37-45

https://doi.org/10.6956/BTDAIS.199806.0037

摘要

傳統的葉綠素測量方法較費工且過程複雜。為尋找簡易、非破壞性的果樹葉片葉綠素含量速測法，本試驗以色差儀（ZE 2000）及葉綠素計（SPAD-502）進行芒果、荔枝及梨葉片葉綠素之測量，並與利用丙酮萃取量比較。結果以色差儀及葉綠素計測值與丙酮萃取量分析比較，其迴歸方程式皆呈二次效應，決定係數都高於0.75，且葉綠素計測值決定係數都高於色差儀。色差儀(a*/b*)2值及葉綠素計讀值與丙酮萃取量比較，決定係數分別為芒果0.88及0.94、荔枝0.93及0.97和梨0.75及0.92。由本試驗之結果，利用葉綠素計及色差儀作為非破壞性估算葉片葉綠素含量時，應考量葉片情形，並以不同生長階段之葉片作檢量線以提高準確性。

關鍵字

葉綠素含量 ； 色差儀 ； 葉綠素計

並列摘要

The objective of this study was to find a rapid and nondestructive method for determination of chlorophyll in fruit tree leaves using mango, litchi and pear as materials. Two nondestructive measure methods were compared with 80% acetone extraction method. The devices used for nondestructive measurement were color meter (model ZE 2000) and chlorophyll meter (model SPAD-502). The results indicated that the regression models express curve relationship, the chlorophyll meter readings correlated well with acetone extraction method. R^2 ＞ 0.92 for mango, litchi and pear leaves. But less the linear relationship was obtained between chromaticity values with color meter and acetone extraction method for pear leaves (R^2=0.75). The color meter and chlorophyll meter can be used for the rapid and nondestructive estimation of leaf chlorophyll content in situ if the number of sampling leaf is enlarged and the distribution of chlorophyll in leaf is homogenous.

並列關鍵字

chlorophyll content ； color meter ； chlorophyll meter