蕎麥品質之研究：GBSS蛋白之初探

The Preliminary Studies on the GBSS Proteins of Buckwheat (Fagopyrium esculentum L. Moench)

張隆仁(Long-Zen Chang) ； 王國津(Gwo-Jhin Wang) ； 謝兆樞(Jaw-Shu Hsieh)

《臺中區農業改良場研究彙報》 70期 (2001/03) Pp. 65-72

https://doi.org/10.6956/BTDAIS.200103.0065

摘要

本研究以蕎麥的種實為材料，進行臘質基因蛋白質層次表現的初探， 首先以SDS-PAGE電泳法及抗血清免疫墨點轉印法分析品種間內胚乳全蛋白、澱粉粒結合蛋白（GBSS）基本特性，以作為日後於分子層次上對蕎麥澱粉特性進一步研究的參考。試驗結果顯示，利用SDS-PAGE以及EITB法分析八個參試的普通種蕎麥品種的種實內胚乳澱粉顆粒時，均可發現在分子量約60 KD的位置出現一個與高粱的臘質蛋白分子量（60 KD）相似的蛋白質，並可以被高粱臘質蛋白的抗血清所辨認。據此，初步推測蕎麥具有與高粱臘質蛋白（waxy protein）高度相似的GBSS蛋白存在。

關鍵字

蕎麥 ； 澱粉粒結合蛋白 ； 臘質蛋白 ； 蛋白抗血清免疫分析

並列摘要

Seed proteins, starch proteins and granule-bound starch synthase (GBSS) proteins were extracted and purified from buckwheat seeds. By using SDS-PAGE and EITB analytical methods, the protein was extracted from seeds of non-waxy sorghum as a check. The results showed that there existed one clearly and densely colored band with molecular weight of 60 KD in eight of common buckwheat cultivars. It was also noticed that there existed a high degree of homology of the 60 KD protein between sorghum and buckwheat. The GBSS protein extracted from buckwheat seeds could be clearly recognizable by the electrophoretical analysis of antiserum (produced by the endosperm starch bounded protein of sorghum as an antigen) by EITB method. It suggested that GBSS protein presented in buckwheat seeds.

並列關鍵字

Buckwheat ； GBSS protein ； Wx-protein ； EITB