基因轉殖番茄檢測技術之研究

The Study of the Detection Technique for Genetically Modified Tomato

楊祐俊(Yu-Chun Yang) ； 陳盈君(Ying-Chun Chen) ； 張隆仁(Long-Zen Chang)

《臺中區農業改良場研究彙報》 94期 (2007/03) Pp. 15-25

https://doi.org/10.6956/BTDAIS.200703.0015

摘要

本研究的主要目的在於建立一個可信賴的基因轉殖番茄檢測技術。透過NCBI資料庫的論文檢索及序列比對，我們找到了一段番茄特有的基因序列　“LAT52　”，針對該序列設計一組正對照檢測引子，測試出適合的反應條件，檢測的敏感度可達到0.5 ng至0.1 ng之間；並且分別對於4種不同的番茄品種以及9種不同的作物進行定性測試，結果發現所有的番茄DNA皆能增幅出預期的片段，證實該引子確實可以做為一個良好的陽性對照組，用來確定DNA品質以及PCR反應條件無誤。除此之外，配合相關研究單位，針對所取得的轉殖番茄材料，我們也成功地建立有效率的複合引子聚合酶連鎖反應檢測技術，節省檢測所需的時間與金錢。

關鍵字

番茄 ； 基因轉殖作物 ； 檢測技術

並列摘要

The purpose of this study was to establish a reliable detection method for transgenic tomato identification. We found a tomato (Lycopersicon esculentum) species-specific gene sequence, LAT52, by means of the paper search and sequence blast at NCBI database. The primer sets were designed as a positive control against this sequence. The suitable condition of PCR reaction was tested and the detection sensitivities were between 0.5 and 0.1 ng of tomato genomic DNA. Qualitative PCR analyses were assayed with 4 different tomato varieties and 9 different plants and those expected amplified products were obtained with all of tomato materials. These results demonstrated the primer set could be utilized to confirm the genomic DNA quality and the PCR condition without mistake. In addition, we have successfully established a multiplex PCR assay technique to detect transgenic tomato material accompany with the related research organization to save the time and money of detection.

並列關鍵字

tomato ； genetically modified crops ； detection technique