應用分子標誌分析番茄種原遺傳歧異度

番茄為世界重要的蔬菜作物，評估所引進核心種原之遺傳歧異度，有助後續育種策略規劃。本研究自國內種苗商蒐集32個番茄種原作為參試材料，調查園藝性狀，並利用分子標誌評估種原間之遺傳歧異度。外表型性狀調查結果顯示，其中20個品系之果實形狀為圓形，12個品系為扁形；而1個品系之植株型態為停心型，14個品系為半停心型，17個品系為非停心型。以16組SSR和4組InDel分子標誌分析結果顯示，增幅的DNA片段大小範圍自100 bp至330 bp，共偵測到71個對偶基因，平均每個基因座有3.6個對偶基因；最高和最低多型性資訊含量為分子標誌T139之0.451及T163與InDel 19之0.334，利用UPGMA親緣樹狀圖與遺傳距離之主座標分析，顯示可將32個種原略分為三群，分別為28個與3個種原，另SF22單一種原為一群。綜上，種原間遺傳歧異度分析結果有助32個番茄種原分類、保管與應用育種工作。

關鍵字

番茄；種原；遺傳歧異度；簡單重複序列

並列摘要

Tomato is one of the most important vegetable crops in the world. Genetic diversity estimation of core germplasm is essential for the further breeding program. In this study, we collected thirty-two tomato germplasm from local seed company and investigated horticultural characteristics. The objective of this study is to evaluate the genetic diversity by using simple-sequence repeats (SSRs) and InDel molecular markers. Phenotypic data of fruit shape revealed 20 were round-shaped lines and 12 were flattened-shaped lines. Investigation of plant habit showed that one line was determinate, 14 lines were semi-determinate and 17 lines were indeterminate. The results of 16 SSR and 4 InDel markers indicated that DNA fragments were ranged from 100 to 330 bp and a total of 71 alleles were detected at an average of 3.6 alleles per marker locus. The highest polymorphic information content (PIC) value was determined as 0.451 for marker T139, whereas marker T136 and InDel 19 had the lowest value of 0.334. The UPGMA cluster analysis grouped 32 tomato genotypes into 3 main clusters. Cluster I and III comprised 28 and 3 genotypes, respectively. A single tomato genotype SF22 was grouped in cluster II. The genetic diversity observed within the 32 tomato genotypes will prove to be of significance for germplasm classification, management, and further utilization in tomato breeding program.

並列關鍵字

tomato ； simple sequence repeat ； genetic diversity ； molecular marke

國際替代計量

應用分子標誌分析番茄種原遺傳歧異度

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