以分子標誌篩選抗黃化捲葉病之番茄種原

番茄栽培期間常因番茄黃化捲葉病而影響果實產量與品質，現行的防治措施無法有效控制病害發生，因此，培育抗病品種為最經濟的防治策略之一。本研究利用已發表的抗病性基因相關之分子標誌，分析雜交組合、自交系與回交組合等單株之抗性基因型。結果顯示分子標誌所增幅之產物，其核苷酸序列可比對至番茄基因組，並與已知的抗性基因序列一致。為了堆疊不同抗性基因或導入抗性基因，進行不同雜交組合，其中3個雜交組合之後裔可成功堆疊Ty-1/Ty-3與Ty-2，1個雜交組合之後裔導入Ty-1/Ty-3，3個F2分離族群堆疊了異質結合的抗性基因型。為篩選優良自交系，進行抗性基因鑑定與園藝性狀調查，選拔出3個帶有同質結合Ty-1/Ty-3之優良純系，可作為後續抗病育種之親本。回交族群之前景選拔，共有6個單株堆疊2個抗性基因，將持續於回交世代選拔同質結合之抗性基因型。

關鍵字

番茄；番茄黃化捲葉病；分子輔助育種

並列摘要

Tomato yellow leaf curl disease (TYLCD) is a severe threat to tomato yield and quality. However, the protection strategies for controlling TYLCD have not been effective so far. Therefore, the development of resistant cultivars against the TYLCD is one of the most effective method to manage the disease. In this study, molecular markers linked to targeted resistant loci were used to screen in several tomato breeding lines. The results showed that the sequences of PCR amplified products were aligned to that of the tomato reference genome and specific loci for the TYLCD resistance. In order to pyramid genes conferring resistance or introduce resistant gene, different hybrids were generated. Ty-1/Ty-3 and Ty-2 were stacked in three F1 populations, Ty-1/Ty-3 was introduced in one F1 population. Three F2 populations were detected containing heterozygous resistant genes. The combined results of identification of resistant genes and evaluation of horticultural traits indicated that three inbred lines harbouring homozygous Ty-1/Ty-3 have potential for release as hybrid parental lines. For foreground selection in backcross breeding, six individuals were pyramiding successfully with two resistant genes, fully homozygous lines in successive generations will be developed.

並列關鍵字

tomato ； tomato yellow leaf curl virus ； marker-assisted selection

國際替代計量

以分子標誌篩選抗黃化捲葉病之番茄種原

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