Field experiment was conducted to study the effect of polyester fabric mulching on the growth of winter production of three cultivars of Eustoma grandiflorum- 'Voyage green', 'Diamond peach' and 'Chu cream'. The results indicate the soil temperature and water content are more stable in mulching treatment than those of in non-mulching. Besides, cut eustoma flower of mulching treatment has better performance on height, leaf area, fresh weight, stem diameter, number of branch, chlorophyll content, flower size and vase life. Furthermore, the number of leaf parch blight of Eustoma grandiflorum significantly decreased by mulching treatment, and the harvest period is shorter and 10 days earlier. As a result, using polyester film mulching can increase cut flower quality and characteristics performance of Eustoma grandiflorm.