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番茄抗黃化捲葉病毒病種原篩選

Screening Tomato Germplasm Resistant to Tomato Yellow Leaf Curl Disease (TYLCD)

摘要


番茄為臺灣重要之果菜類蔬菜,栽培時常因番茄黃化捲葉病毒(Tomato yellow leaf curl virus, TYLCV)誘發之番茄黃化捲葉病毒病(Tomato Yellow Leaf Curl Disease, TYLCD),造成嚴重之產量及品質下降。臺灣番茄抗TYLCD育種皆以黑柿及小果番茄為研究重心,因此其對於TYLCD之抗病性較強,然全紅番茄品種多為國外進口且不耐TYLCD,故幾乎無法於露地大規模栽種。本研究目標為選育適合於臺灣栽種且抗番茄黃化捲葉病毒之全紅番茄品種,於2015年自國內、外蒐集番茄53個品種,並於亞蔬-世界蔬菜研究中心利用銀葉粉蝨接種TYLCV,進行種原抗病性檢定。所有品種於接種病毒後14天皆可於葉片中檢測出病毒DNA,其中11個番茄品種於接種後28天並無病徵表現,移植田間後生長發育及結果皆表現正常,又以帶有Ty1及Ty3抗病基因之番茄品種之抗病性最佳,未來將作為番茄抗TYLCD育種之重要親本材料。

並列摘要


Tomato is one of the important vegetables in Taiwan. However, the Tomato yellow leaf curl disease (TYLCD) caused by Tomato yellow leaf curl virus (TYLCV) often significantly reduce the yield and quality of tomato cultured in Taiwan. This research aimed to breeding the native red tomato variety that can resistant to tomato yellow leaf curl virus in Taiwan. There are 53 tomato cultivars that was collected from our country and abroad in 2015. There are 11 tomato lines showing no symptoms development after infected TYLCTHV by whitefly at 28 Days after inoculation. Tomato cultivars with Ty1 and Ty3 gene showed high resistant ability. The virus was detected in all test plant even the plants have not any symptom development. These 11 lines can be used as materials for tomato heat tolerance and TYLCD resistant breeding program in the future.

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