Determination of Plant Bioactive Compounds. Antioxidant Capacity and Antimicrobial Screening

Charalampos Proestos ； Panagiotis Zoumpoulakis ； Vassileia J. Sinanoglou

《Focusing on Modern Food Industry》 2卷1期 (2013/02) Pp. 26-35

並列摘要

It is well known that bioactive compounds such as polyphenols are constituents of many plants and herbs, and they have attracted a great deal of public and scientific interest because of their health-promoting effects and of their potential applications in foods as antioxidants and antimicrobial agents. Selected plants of interest in traditional diets were examined in order to determine their phenolic composition, antioxidant capacity and antimicrobial activity. Reversed phase high performance liquid chromatography coupled with DAD was employed for the identification and quantification of polyphenols. The phenolic substances were identified and quantified after comparison with reference standards. Catechin, ferulic acid and quercetin were the main polyphenol compounds. The antioxidant capacity of the extracts and of the isolated polyphenol compounds were determined with the Rancimat test using sunflower oil as substrate (the PF factor was determined), the FRAP assay and the DPPH assay (IC_(50)). All plants and isolated compounds exhibited antioxidant capacity. The antimicrobial activity of the extracts against selected microorganisms was also investigated using the disc diffusion method. The plants Pelargonium purpureum and Sideritis scardica exhibited the most potent antimicrobial activity against all selected pathogenic microorganisms such as E. coli and L. monocytogenes. These results suggested that the plants selected for this study had properties that required attention for their potential health effect and for possible applications in food products.

並列關鍵字

Phenolic Antioxidants ； RP-HPLC ； FRAP Assay ； DPPH ； Rancimat Test ； Antimicrobial Activity