- 期刊
評估厭氧檢體輸送管CMP^(TM) Anaerobic TranSwab對厭氧菌之保存效能
Evaluation the Efficacy of CMPTM Anaerobic TranSwab for Use in the Preservation of Anaerobic Bacteria
摘要
為評估厭氧檢體輸送管CMP^(TM) Anaerobic TranSwab（啟新生物科技有限公司，台灣）之菌種保存效能，吾等參考CLSI之微生物輸送系統之規範M40-A（2003年）做為評估方法，選用厭氧菌Bacteroides fragilis（ATCC 25285）與Propionibacterium acnes（ATCC 6919）進行測試，將約10^6 CFU之菌量接種於棉棒後插入厭氧輸送裝置的培養基中，同時為模擬檢體不同的輸送條件，分別將CMP^(TM) Anaerobic TranSwab保存於室溫及4℃環境。經過0、24及48小時後以生理食鹽水回收，取回收菌液進行十倍連續序列稀釋，然後取各稀釋液100L塗抹於平板培養基Brain-heart infusion agar，培養在厭氧操作培養箱（含80%N2，10%CO2及10%H2之混合氣）48小時之後，選取3000 CFU生長菌落之平板培養基進行菌落計數，再依稀釋度換算成總菌量，然後將各不同時間點回收之總菌量與0小時之總菌量做比較。依據CLSI之標準：在4℃環境之下，菌量對數值下降（log reduction）不大於3，上升值不大於1；在室溫環境之下，則菌量對數值下降不大於3。本試驗結果顯示，CMPTM Anaerobic TranSwab在24及48小時之保存時間無論是在室溫或4℃環境下皆能夠有效保存B. fragilis及P. acnes，符合CLSI M40-A規範之要求。本試驗結果將可供作臨床微生物實驗室選用厭氧檢體輸送管的參考。
關鍵字
厭氧檢體輸送管並列摘要
The efficiency of bacteria preservation of CMP^(TM) Anaerobic TranSwab (Creative Biotechnology Co., Ltd. Taiwan.) was evaluated according to the CLSI approved standard M40-A ＂Quality control of microbiological transport systems＂ (2003). The experiments used anaerobic clinical bacteria, Bacteroides fragilis and Propionibacterium acnes, to evaluate the efficacy of CMP^(TM) Anaerobic TranSwab. After inoculated 10^6 CFU organisms on the CMP^(TM) TranSwab, the CMP TM TranSwab were preserved in room temperature or at 4℃ to simulate how the clinical specimen is transported. The survived bacterium were recovered by saline after 0, 24 and 48 hours storage. The recovered bacterial suspensions were performed in a ten-fold serial dilution method, and then plating with spreading method and counting the number of the survival bacteria after incubating at suitable conditions. According to CLSI M40-A statement: ＂In room temperature, there should be no more than a 3 log decline in CFU between the zero-time CFU count and the CFU of the swabs that were stored at different time interval; at 4℃, there should be no more than a 1 log increase in CFU and no more than a 3 log to decline in CFU between the zero-time CFU and the CFU of the swabs that were stored at different time interval.＂ The results of this study showed that CMP^(TM) Anaerobic TranSwab can well preserved the B. fragilis and P. acnes up to 48 hours whether stored in room temperature or at 4℃. It fulfills the CLSI M40-A guideline. The experiment result could be act as reference data when Clinical Microbiology Laboratory choosing their transport device.
延伸閱讀
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