抗氧化複合組成物配方與CCl_4誘導大鼠肝炎模式中的護肝功能評估

Hepatoprotective Activity of a Specialized Formulation Containing Novel Antioxidants on CCl4-Induced Rat Chronic Liver Damage Model

徐瑞霞(Jui-Hsia Hsu) ； 陳勁初(Chin-Chu Chen)

《檢驗及品保雜誌》 6卷4期 (2017/11) Pp. 186-197

https://doi.org/10.6573/JTQA.201711_6(4).04

摘要

本試驗探討由紫丁香蘑(Lapista nuda)菌絲體萃取物、金針菇(Flammulin avelutipes)萃取物、N-乙醯基-D-葡萄糖胺、蔓越莓萃取物、洛神花萼萃取物、穀胱甘肽、米胚芽萃取物（含神經醯胺）及維生素C等組成之抗氧化複合組成物配方（以下簡稱組合物, BD）對四氯化碳誘發大鼠慢性肝炎的保護功效。四氯化碳(20%；0.2 mL/100 g body weight)每星期投予兩次，共八星期。大鼠於四氯化碳投予前一天，每日經口投予組合物一次，直至實驗終了。組合物對第1、3、6及8週四氯化碳所升高的血漿alanine aminotransferas (ALT)及aspartate aminotransferas(AST)值有明顯降低作用。此外，四氯化碳誘發大鼠慢性肝炎於第8週血漿白蛋白值下降，組合物可增加血漿白蛋白含量。四氯化碳誘發大鼠慢性肝炎明顯增加肝臟、脾臟重量，組合物可以減輕肝臟、脾臟重量。四氯化碳誘發慢性肝炎會減少肝臟蛋白質及glutathione含量、增加肝臟含水量、脂質過氧化程度及膠原蛋白含量，組合物明顯增加肝臟蛋白質及glutathione含量，及降低肝臟含水量、脂質過氧化程度和膠原蛋白含量。而投予組合物亦明顯增加肝臟抗氧化酵素SOD及GSH-Px的活性。更重要地，肝臟病理檢查結果顯示組合物能減輕四氯化碳所誘發的肝臟損傷及纖維化程度。由這些結果明確顯示，此配方組合物可以減輕四氯化碳所誘發的大鼠慢性肝炎。

關鍵字

液態發酵 ； 慢性肝病 ； 護肝 ； 抗氧化 ； 紫丁香蘑菌絲體萃取物

並列摘要

This study explored the protective effect of a specialized formulation containing novel antioxidants (BD) on carbon tetrachloride-induced chronic liver damage in rats. Carbon tetrachloride (CCl_4; 20%, 0.2 mL/100 g body weight) was administered twice a week for eight weeks. Rats were given the formulation daily before the administration of CCl_4, until the end of the experiment. The formulation significantly reduced the levels of elevated plasma ALT and AST values induced by CCl_4 on the first, third, sixth and eighth weeks. In addition, the CCl_4-induced decrease in plasma albumin concentration was signicantly inhibited by administration of the formulation at week 8. Moreover, the formulation significantly reduced the weight of liver and spleen induced by CCl_4. The formulation could alsosignificantly elevatedthe CCl_4-induced decrease in liver protein and glutathione levels, as well as reducingthe water content, lipid peroxidation and collagen content of the liver. Furthermore, treatment wit h the formulation significantly increased the activities of antioxidant enzymes SOD and GSH-Px in the liver. Last but not least, the formulation reduced liver damage and the amount of histologically detectable fibrosis produced by CCl_4. From these results, it is clear that supplementation with this formulation could alleviate the malfunctions of liver among rat groups hepatic damaged with CCl_4.

並列關鍵字

Submerged liquid fermentation ； chronic liver disease ； liver protecting ； antioxidant activity ； Lepista nuda extract