評估生殖道定植菌在CMP^(TM) GBS運送增菌培養管不同培養環境的消長

Growth Enhancement or Suppression of Genital Tract Colonizers on CMP^(TM) GBS TransCultSwab in Different Incubation Conditions

黃玉霞(Yuxia Huang) ； 蔡文城(Wen-cherng Tsai)

《檢驗及品保雜誌》 7卷3期 (2018/07) Pp. 111-118

https://doi.org/10.6573/JTQA.201807_7(3).01

摘要

CMP^(TM) GBS TransCultSwab（GBS運送增菌培養管；簡稱GBS運送管），可快速、便捷及高效地篩檢B群鏈球菌(Group B streptococcus，GBS，Streptococcus agalactiae)，GBS可呈現胡蘿蔔色，特異性達100%。臨床上採集的孕婦陰道或直腸分泌物檢體包含多種生殖道定植菌，為了研究各種微生物在GBS運送管24 h內的生長變化，本研究選取7種試驗菌，包括GBS及金黃色葡萄球菌(Staphylococcus aureus)、白色念珠菌(Canidia albicans)、大腸埃希氏菌(Escherichia coli)、枯草芽孢桿菌(Bacillus subtilis)、糞腸球菌(Enterococcus faecalis)、棒狀桿菌(Corynebacterium mycetoides)。單獨或混菌接種至GBS運送管於一般35℃培養箱，培養0, 2.5, 5.0, 7.5, 16及24小時，結果發現以GBS生長速度最快，其餘依次是E. faecalis、S. aureus與C. albicans，B. subtilis和C. mycetoides生長量的變化不明顯，而E. coli則明顯受到抑制。當GBS與除E. faecalis 外的五種測試菌的混合液接種至GBS運送管，GBS仍可快速生長，並且顯色。但當E. faecalis存在（GBS與糞腸球菌比例為10:1）七種測試菌中，吾等發現因E. faecalis的存在，GBS的生長及顯色能力稍微受到干擾，培養至16 h時，並不能觀察到明顯的胡蘿蔔色，而須繼續培養至約24 h後才能明顯看到顯色。另外，無論是接種純菌還是混合菌液至GBS運送管，置於室溫培養24 h，所有測試菌均無明顯消長。綜合上述，吾等認為採集孕產婦陰道及/或直腸分泌物後，儘快將GBS運送管放入35℃培養箱中或檢驗室收到檢體後立即置入35℃培養箱，至少5小時後移種至適當培養基，將可有效提高GBS的檢出率，降低檢驗的人力物力成本，並提早發出最終報告，值得檢驗室廣泛應用。

關鍵字

B群鏈球菌 ； GBS運送增菌培養管 ； 生殖道定植菌

並列摘要

The CMP^(TM) GBS TransCultSwab is a rapid, convenient, and highly effective device for screening group B streptococci (GBS, Streptococcus agalactiae) with 100% specificity. GBS colonies appear carrot-red on GBS TransCultSwab. Vaginal or rectal swab specimens from pregnant woman usually contain many genital tract colonizers. To evaluate the effectiveness of GBS TransCultSwab in the screening of GBS, we tested seven microorganisms including GBS, Staphylococcus aureus, Canidia albicans, Escherichia coli, Bacillus subtilis, Enterococcus faecalis, and Corynebacterium mycetoides. They were inoculated individually or in combination on GBS TransCultSwab and incubated at 35℃ for 0, 2.5, 5.0, 7.5, 16, and 24 hours. GBS was found to be the fastest growing bacteria on GBS TransCultSwab, followed by E. faecalis, S. aureus, and C. albicans. The growth of B. subtilis and C. mycetoides was not changed, but that of E. coli was inhibited significantly on GBS TransCultSwab. When GBS was mixed with 5 other test organisms (except E. faecalis), it still grew rapidly to form orange-red colonies. The presence E. faecalis (at the ratio of GBS to E. faecalis of 10:1) in the mixture of all 7 test organisms was found to slow down the growth of GBS on GBS TransCultSwab as orange-red colonies were not significantly observed until 24 hours of incubation. Regardless of being inoculated alone or in combination with other organisms, all test microorganisms showed no significantly increase or decrease in quantity on GBS TransCultSwab at room temperature. Taken together, we recommend that the GBS TransCultSwab be incubated at 35℃ immediately after it is inoculated with vaginal or rectal specimens or as soon as it is transported and received by the laboratory. A 5-hour or longer incubation time at 35℃ would undoubtedly increase the isolation rate of GBS, reduce the costs of manpower and laboratory consumables, and shorten the turnaround time.

並列關鍵字

Streptococcus agalactiae ； CMP^(TM) GBS TransCultSwab ； genital tract colonizers