3D printer, open resources and Maker movement have brought new learning content for technical and vocational education. This study explores whether gender differences exist in Maker curriculum achievement. Three comprehension tests are developed, including Arduino test, 3D printing test, and 3D modeling test. Technical test score is based on the performance of the Maker work, line-tracking autonomous car. According to the results, it is concluded that there is no significant difference between male and female students in all four tests. In the future, we would like to promote the curriculum to other domains for a better understand about gender difference in Maker education.