單寧酵素處理白芍之抗氧化活性研究

Study on antioxidant activities of enzyme hydrolysis Paeonia lactiflora Pall

陳惠欣(Hui-Hsin Chen) ； 謝衣鵑(I-Chuan Sheih) ； 方信裕(Hsin Yu Fang) ； 盧明傑(Min-Jer Lu)

《華醫學報》 52期 (2020/06) Pp. 37-50

摘要

白芍（Paeonia lactiflora Pall.）分佈在台灣南部，在中國廣泛種植。白芍的莖在東方醫學中長期以來一直用於治療腸炎，痢疾和瘙癢。本研究目的主要藉由單寧酶（Tannase）處理白芍乙醇萃取物來探討其化學組成和生理活性。白芍經乙醇萃取25℃，24小時後，在用單寧酶37℃反應30分鐘。評估其抗氧化能力包括DPPH清除能力、超氧離子、總還原力（TEAC）、氫過氧化物和金屬離子的結合力。結果水解後的白芍在200ppm超氧離子、氫過氧化物、DPPH清除能力是92%，85%，65%明顯較未水解白芍液為高。在濃度為100ppm時總還原力（TEAC）結果顯示水解後的白芍較高（2.4%vs.1.0%）。同時在銅離子和鐵離子比較上，結果顯示水解後的白芍較高（42.6/33.5%vs.14.6/7.6%）。在RAW 264.7細胞毒性測試經144小時結果顯示水解後的白芍細胞存活較高。以上結果證明水解後的白芍分子沒食子酸後能有效提升抗氧化活性，對毒性影響較低。

關鍵字

白芍 ； 單寧酶 ； 抗氧化活性

並列摘要

Paeonia lactiflora Pall. is distributed in southern Taiwan and widely cultivated in China. The stems of Paeonia lactiflora Pall. have long been used for the treatments of enteritis, dysentery and itch in oriental medicine. Investigations were conducted to the effects of tannase-modify Paeonia lactiflora Pall. on the chemical compositions and biological activities. Paeonia lactiflora Pall. stems were extracted with ethanol at 25 ℃ for 24 hours, and the Paeonia lactiflora Pall. was hydrolyzed with tannase at 37 ℃ for 30 mins. The antioxidant capacity of antioxidants in vitro was evaluated by using the assays of DPPH, superoxide anion, TEAC, hydrogen peroxide, and chelating metal ions. The results show that tannase-treated Paeonia lactiflora Pall has a higher antioxidant capacity than the untreated analog at a concentration of 200 ppm, with greater superoxide anion, hydrogen peroxide and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging effects (92%, 85% and 65%, respectively). At a concentration of 100 ppm, the tannase-treated Paeonia lactiflora Pall also had significantly higher TEAC antioxidant activity compared to the untreated Paeonia lactiflora Pall (2.4 % vs. 1.0 %). Furthermore, at 150 ppm the chelating activity of the treated Paeonia lactiflora Pall. for Fe^(+2)/Cu^(+2) ions was higher compared to the untreated (42.6/33.5 % vs. 14.6/7.6 %, respectively). The viability of RAW 264.7 cells incubated with tannase-treated Paeonia lactiflora Pal. for 144 h was higher compared to analogs incubated with untreated Paeonia lactiflora Pal. Our results demonstrated that antioxidant activity in flavonoids varies, and that the presence of galloyl residues decreased the antioxidant activity of Paeonia lactiflora Pal., and more low toxicity effects.

並列關鍵字

Paeonia lactiflora Pall ； Tannase ； Antioxidant capacity