Applications of NGS on Mitochondrial DNA Analysis for Forensic Samples

Chia-Hung Huang ； Tsun-Ying Huang ； Fang-Chen Chung ； Chu-Chun Hsu ； Guan-Cheng Peng ； Yi-Ting Chang ； Chun-Yen Lin

《Forensic Science Journal》 16卷1期 (2017/12) Pp. 51-57

https://doi.org/10.6593/FSJ.2017.1601.05

摘要

Sequencing for forensic DNA identification is usually performed by using the Sanger method with capillary electrophoresis presently. However, it still has some problems by using this system such as determinations of the number of C for C-stretch and the sequence heteroplasmy in mitochondrial DNA D-loop region used in forensic identification. The new technology NGS is with the potential to resolve these problems. In this study, sequencing of the mitochondrial DNA for 39 forensic samples were successfully performed by NGS. Three samples of them were observed with the sequence heteroplasmy but not observed from sequencing by the Sanger method with capillary electrophoresis. Totally there were 14 types of C-stretch for HV1 and HV2 combinations. The results showed the feasibility for applications of NGS on mitochondrial DNA analysis for forensic samples. This is the first report for sequencing the forensic samples by using NGS in Taiwan.

關鍵字

Forensic Science ； Capillary Electrophoresis ； Next-Generation Sequencing (NGS) ； Mitochondrial DNA (mtDNA)