固定相48:＂襪－球＂層析醛醌化合物在C_(60)碳簇固定相上之滯留行為研究

Stationary Phases 48(1): ＂Sock.Ball＂ Liquid Chromatography of Aldehydes, Ketones and Quinones on C_(60)-fullerene Stationary Phase

張勝雄(Cheng-Shyong Chang) ； 鄧朝岡(Tschau-Gan Den) ； 陳成章(Cheng-Chang Chen)

《化學》 54卷4期 (1996/12) Pp. 7-19

https://doi.org/10.6623/chem.1996056

摘要

本文以自製C_(60)碳簇固定相，在逆相層析中對醛酮醌類及芳香化合物進行層析測試，並與商用RP-18固定相所測出之層析結果做比較，結果顯示自製之C_(60)碳簇固定相在分離醛酮醌化合物及芳香化合物與商用RP-18固定相有不同的滯留行為，在逆相層析中此產品對於醛酮醌化合物有良好之特殊分離效果，其中對於各種構型之蒽醌類鋰蒽酮類衍生物，可藉由化合物與C_(60)碳簇固定相間之＂電荷轉移＂及＂襪球＂結合等作用，將此類化合物作選擇之分離，例如，類似襪狀構型Acylamide yellow蒽醌衍生物與C_(60)碳簇固角定相上之C_(60)官能基有最強之donor-acceptor電荷轉移吸附作用，此種特殊之吸附關係以＂襪－球＂結合模式最佳、其次是彎曲型、再其次是平面型，結果顯示C_(60)-fullerene-phase固定相在不同構型之蒽醌類或蒽酮類衍生物分析鑑定上比冏用RP-18固定相提供了較好之形狀選擇特性，且此種滯留功能可成功的應用在崑醌類顏料及萘醌天然物之分離上。

關鍵字

高效液態層析 ； C_(60)碳簇固定相 ； “襪-球”層析 ； 醛酮醌化合物

並列摘要

Aldehydes, ketones or quinones were tested and separated using a prepared C_(60)-fullerene stationary phase or a commercial RP-18 phase with methanoVwater (100/0-80/20) as mobile phase. The results indicate that C_(60)-fullerene phase has the special selectivities and good performance for the separation of such solutes as compared with those of RP-18 under the same reversed phase conditions. Base on the mechanism of electron donor-acceptor interaction or ＂sock-ball combination＂ interaction, quinones or some ketones can undergo more pronounced interaction with CGO ligand. For example, the tested Acrylamide yellow, a sock-like structure of quinone, calculated by means of semiempirical method, can undergo a ＂sock-ball＂ liquid chromatography with C_(60)-fullerene phase, and possesses the strongest retention. Other quinones with bend or planar structure eluted on C_(60)-fullerene phase can undergo a chargetransfer liquid chromatography, but only possess strong retentions. This retention power can be applied successfully to separate some quinone pigments or natural products.

並列關鍵字

HPLC ； C_(60)-fullerene-phase ； ＂Sock-Ball＂ Liquid Chromatography ； Aldehydes ； Ketones ； Quinones