本文以自製C_(60)碳簇固定相,在逆相層析中對醛酮醌類及芳香化合物進行層析測試,並與商用RP-18固定相所測出之層析結果做比較,結果顯示自製之C_(60)碳簇固定相在分離醛酮醌化合物及芳香化合物與商用RP-18固定相有不同的滯留行為,在逆相層析中此產品對於醛酮醌化合物有良好之特殊分離效果,其中對於各種構型之蒽醌類鋰蒽酮類衍生物,可藉由化合物與C_(60)碳簇固定相間之"電荷轉移"及"襪球"結合等作用,將此類化合物作選擇之分離,例如,類似襪狀構型Acylamide yellow蒽醌衍生物與C_(60)碳簇固角定相上之C_(60)官能基有最強之donor-acceptor電荷轉移吸附作用,此種特殊之吸附關係以"襪-球"結合模式最佳、其次是彎曲型、再其次是平面型,結果顯示C_(60)-fullerene-phase固定相在不同構型之蒽醌類或蒽酮類衍生物分析鑑定上比冏用RP-18固定相提供了較好之形狀選擇特性,且此種滯留功能可成功的應用在崑醌類顏料及萘醌天然物之分離上。
Aldehydes, ketones or quinones were tested and separated using a prepared C_(60)-fullerene stationary phase or a commercial RP-18 phase with methanoVwater (100/0-80/20) as mobile phase. The results indicate that C_(60)-fullerene phase has the special selectivities and good performance for the separation of such solutes as compared with those of RP-18 under the same reversed phase conditions. Base on the mechanism of electron donor-acceptor interaction or "sock-ball combination" interaction, quinones or some ketones can undergo more pronounced interaction with CGO ligand. For example, the tested Acrylamide yellow, a sock-like structure of quinone, calculated by means of semiempirical method, can undergo a "sock-ball" liquid chromatography with C_(60)-fullerene phase, and possesses the strongest retention. Other quinones with bend or planar structure eluted on C_(60)-fullerene phase can undergo a chargetransfer liquid chromatography, but only possess strong retentions. This retention power can be applied successfully to separate some quinone pigments or natural products.