藏紅T與核酸的超分子作用機理研究

Mechanism Studies on the Supramolecular Interactions of Safranine Twith Nucleic Acids

黃承志(Cheng-Zhi Huang) ； 李原芳(Yuan Fang Li) ； 劉紹璞(Shao Pu Liu) ； Xi-Dong Liu

《化學》 56卷1期 (1998/03) Pp. 25-30

https://doi.org/10.6623/chem.1998004

摘要

研究了在生理酸度下，陽離子碱性染料藏紅T(ST)與核酸的超分子作用。當ST與核酸的莫爾比低於0.17時，ST嵌入雙鏈核酸的碱基對之間，嵌入的莫爾比為DNA分子上每個核苦酸殘基結合一個ST分子，而RNA分子上每兩個核苦酸殘基結合一個ST分子，結合常數都達10^3級。很據所測定之結合常數計算了在12˚C時作用過程的複合自由能變，複合反應捨和復合反應熵。

關鍵字

核酸 ； 藏紅T ； 超分子作用 ； 分子吸收光譜

並列摘要

This paper describes the supramolecular interactions of safranine T with nucleic acids at physiological acidity. When the molar ratio of ST to nucleic acids is less than 0.17 、S T is intercalated in the double strands of nucleic acids by the ratio of each nucleotide residue of DNA to a molecule of ST. and by the ratio of two nucleotide residue of RNA to a molecule of ST. Binding constant is about 103 order. The change of free energy I enthalpy and entropy was calculated for the supramolecular interactions according to the binding constant determined.

並列關鍵字

Nucleic acids ； Safranine T ； Supramolecular interaction ； Molecular absorption spectroscopy