M-199與NCSU-23培養液對豬卵母細胞體外成熟與後續胚發育之影響

Effects of M-199 and NCSU-23 Media on the in Vitro and Further Development of Porcine Oocytes

林育安(Yu-An Lin) ； 廖明輝(Ming-Huei Liao) ； 陳銘正(Ming-Cheng Chen)

《弘光學報》 57期 (2009/12) Pp. 91-99

https://doi.org/10.6615/HAR.200912.57.07

摘要

本試驗之目的，乃研究M-199與NCSU-23 (North Carolina University 23 Medium)培養液對豬卵母細胞體外成熟與後續胚發育之影響。卵丘-卵母細胞複合體 (cumulus-oocyte complexes, COCs) 分別培養於M-199或NCSU-23成熟培養液中48小時後，將部分去除卵丘之卵母細胞固定，檢查成熟率；其餘進行體外受精，隨後並培養於NCSU-23胚培養液中16小時與144小時，分別檢查其受精與胚發育情形。試驗結果顯示，M-199 組與 NCSU-23 組之成熟率、受精率與雄原核形成率，彼此間無顯著差異，但NCSU-23組之胚發育之比率高於M-199 組(P＜0.05)。我們試驗結果證實，NCSU-23 培養液較M-199培養液有利於後續胚之發育。

關鍵字

體外受精 ； 體外成熟 ； 豬卵母細胞

並列摘要

The objective of this study was to investigate the effects of the M-199 and NCSU-23 media (North Carolina University 23 Medium) on the in vitro and further development of porcine oocytes. All oocyte-cumulus complexes (COCs) were cultured in M-199 or NCSU-23 medium for 48 hours, respectively. After maturation cultured, Some cumulus-free oocytes were fixed the maturation was examined the maturation rate, and the rest proceeded to fertilization in vitro. Then, the inseminated oocytes were transferred into the NCSU-23 embryo medium for 16 hours and 144 hours incubation and then fixed oocytes and examined under a phase-contrast microscope, respectively. The results showed that there was no difference between the maturation rate, fertilization rate and male pronucleus formation rate of M-199 and NCSU-23 group. However, the embryo developmental capacity of NCSU-23 group was higher than M-199 group (P＜0.05). Our results demonstrated that NCSU-23 medium was beneficial for embryo development than M-199.

並列關鍵字

in vitro fertilization ； in vitro maturation ； porcine oocyte

參考文獻

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