Development of antioxidant activity and pattern recognition of terminalia chebula retzius extracts and its fermented products

訶子萃取物與發酵產物的抗氧化活性與型態辨識之開發

Chia-Lin Chang ； Che-San Lin

《弘光學報》 61期 (2010/12) Pp. 115-129

https://doi.org/10.6615/HAR.201012.61.08

摘要

這項研究是確定訶子未發酵萃取物和發酵產物之間的抗氧化活性差異，以及辨識抗氧化型態。依據清除1,1-diphenyl-2-picrylhydrazyl radical (DPPH)作用試驗與horseradish peroxidase (HRP)-luminol-hydrogen peroxide (H_2O_2)試驗分別分析訶子甲醇萃取物、水萃取物、95%乙醇萃取物、乾燥粉末在25°C下發酵產物及95%乙醇萃取後殘渣在37°C下發酵產物顯示具有好的抗氧化活性；依據pyrogallol-luminol試驗分析訶子甲醇萃取物、水萃取物、95%乙醇萃取物、乾燥粉末在15°C下發酵產物及水萃取物在25°C下發酵產物皆顯示出好的抗氧化活性。抗氧化型態圖譜顯露出有價值的資訊，而且顯示清除DPPH作用試驗與HRP-luminol-H_2O_2試驗之間有良好的關聯性，未來這些結果可能代表著有希望的醫藥植物來源。

關鍵字

抗氧化活性 ； 抗氧化型態 ； 未發酵萃取物 ； 發酵產物 ； 訶子

並列摘要

This study was to determine the difference of antioxidant activities between unfermented extracts and fermented products for Terminalia chebula Retzius, and to recognize antioxidative patterns. The methanol extract, water extract, 95 % ethanol extract, fermented product of dried powder at 25 °C and fermented product of residues after 95 % ethanol extraction at 37 °C showed good antioxidant activities based the scavenging effect on 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assay and the horseradish peroxidase (HRP)-luminol-hydrogen peroxide (H_2O_2) assay, respectively. The methanol extract, water extract, 95 % ethanol extract, fermented product of dried powder at 15 °C and fermented product of water extract at 25 °C exhibited a good value of antioxidant activity based on the pyrogallol-luminol assay. The antioxidative pattern plots revealed valuable information and showed good correlation between scavenging effect on DPPH radical assay and HRP-luminol-H_2O_2 assay. These results may represent promising plant-sources of medicine in the future.

並列關鍵字

Antioxidant activity ； antioxidative pattern ； unfermented extract ； fermented product ； Terminalia chebula Retzius

參考文獻

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