- 期刊
Leukotriene C_4 biosynthesis in isolated August rat peritoneal leukocytes
摘要
THE mixed leukocyte population obtained from the peritoneum of the August rat is a potentially important experimental model of inherent eosinophilia that has not been well characterized. In the present study, isolated cell preparations generated a concentration-dependent release of leukotriene (LT) C_4 when exposed to the Ca^(2+) ionophore A23187, reaching maximal stimulation at 5.0 μM. This response was inhibited by the 5- lipoxygenase activating protein antagonist MK- 886 (0.1 μM), nominally Ca^(2+) and Mg(2+)-free incubation media and by activation of protein kinase C via phorbol 12-myristate 13-acetate (50nM). These findings establish a model system for investigating LTC_4 profiles contingent with innate peritoneal eosinophilia and are consistent with the hypothesis that cellular LTC_4 biosynthesis is phosphoregulated.
延伸閱讀
- 莊秀琪、蘇秀琪、劉昌宇、楊天樹（1996）。Dietary Modulation on Peptidyl-leukotriene Production from Porcine Lung Tissue。中華民國獸醫學會雜誌，22(6)，381-393。https://doi.org/10.7009/JTCSVS.199612.0381
- Ratthé, C., Ennaciri, J., Gonçalves, D. M. G., Chiasson, S., & Girard, D. (2009). Interleukin (IL)-4 Induces Leukocyte Infiltration In Vivo by an Indirect Mechanism. Mediators of Inflammation, 2009(), 119-128. https://doi.org/10.1155/2009/193970
- Tchorbanov, B., Marinova, M., & Grozeva, L. (2011). Debittering of Protein Hydrolysates by Lactobacillus LBL-4 Aminopeptidase. Enzyme Research, 2011(), 564-570. https://doi.org/10.4061/2011/538676
- Oosterhout, A. J. M. V., Poel, A. V. D., Koenderman, L., Roos, D., & Nijkamp, F. P. (1994). Interleukin-5 potentiates sulfidopeptide leukotriene production by human eosinophils. Mediators of Inflammation, 1994(), 53-55. https://doi.org/10.1155/S0962935194000098
- Dupont, G. D., Croisier, J. L., Camus, G., Brumioul, D., Hartert, M. M., Sondag, D., Deby, C., & Lamy, M. (1994). Inactivation of α_2-macroglobulin by activated human polymorphonuclear leukocytes. Mediators of Inflammation, 1994(), 117-123. https://doi.org/10.1155/S0962935194000141