Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties

Ivo Lebrun ； Valeria Cavallaro ； Luiz Juliano ； Maria A. Juliano ； Maria C. C. de Sousa e Silva

《Mediators of Inflammation》 2004卷 (2004/12) Pp. 263-268

https://doi.org/10.1080/09629350400003068

摘要

CASEIN, a protein found in milk of several species, is divided into different chains from 19 to 25 kDa. Casein is also considered as a source of amino acids and generating peptides with biological activities such as opiate, immunostimulating, antibacterial, peptidase inhibitors, among others. In this work, Sephadex G-10 chromatography followed by high-performance liquid chromatography isolation purified NZCase TT, an industrial culture media for tetanus toxin production. In the first step, four pools were isolated and tested in different assays: isolated smooth muscle assay (guinea pig ileum, rat uterus), phagocytosis in vitro of opsonized sheep red blood cells, and hydrogen peroxide (H_2O_2) release from mouse peritoneal macrophages. Pool III was the main active pool being able to potentiate bradykinin action in guinea pig ileum, stimulating phagocitic activity by resident macrophages and increasing H_2O_2 release from macrophages previously activated with bacille Calmette Guerin. Using mass spectra the primary structure of the main peptide from pool III was obtained-INKKI, which corresponds to β-casein fragment 26-30. The immunostimulating action is probably related to a direct action in macrophage cells.

關鍵字

Mastoparan ； Casein peptides ； Inflammation ； Macrophages ； Peptide isolation