The in vitro cytotoxicity of five fractions derived from the crude water-soluble extract (CWSE) of Momordica charantia, a known medicinal herb was tested on four cancer cell models; MDA-MB 436 and 231 (breast cancer cell lines), HeLa (Cervical cancer cell lines) and A549 (Lung cancer cell lines). Nhexane (D4) < Dichloromethane (D5) < Ethyl acetate (D3) < Butanol (D2) < Aqeous (D6) fractions, all obtained via solvent partitioning in their order of increasing polarity were administered at varied concentrations (50, 75,100, 125, 150 and 200 μg/mL) for 24 h. Cell viability was detected using the MTT assay. Cells treated with the Butanol and Aqueos fractions respectively showed survival rates as high as 96.11% and 85.75% for MDA-MB 231; 93.59% and 88.28% for MDA-MB 436; 101.62% and 96.76% for HeLa and 89.55% and 81.40% for A549 even at 200μg/mL, the highest considered concentration. Whereas, at tested concentrations, cells treated with the N-hexane, Dichloromethane and Ethyl acetate fractions showed marked cytotoxic effects with the Inhibitory concentration (IC_(50)) ranging between 100μg/mL and 125μg/mL. We also observed that the ethylacetate fraction displayed the highest cytotoxicity having its approximate IC_(50) at 100μg/mL, especially for MDA-MB 231 (~51%), HeLa (~54%) and A549 (~43%). The percentage viability for MDA-MB 436 at 100μg/mL is about 64.2%, albeit at 125μg/mL percentage viability dropped to 34.7% This paper may provide cytological evidence for the inhibitory effect of Momordica charantia on certain cancer cell proliferation.