In vitro antibacterial activity of Tagetes minuta and Capsicum frutescens extracts against pectobacterium carotovorum

Nyamari Jackline Kwamboka ； Wolukau Joseph Ngwela ； Gesimba Robert Morwani

《International Journal of Agricultural Sciences》 6卷8期 (2016/08) Pp. 1119-1127

摘要

Pectobacterium carotovorum bacteria cause soft rot and vascular wilt of vegetables in the field and post harvest decay. The aim of this study was to determine in vitro antibacterial activity of Tagetes minuta and Capsicum frutescens extracts against Pectobacterium carotovorum. Aqueous extracts of T. minuta and C. frutescens were tested against Pectobacterium by modified disc diffusion method on agar plates and potato chips assay. Streptomycin sulphate and distilled water were the positive and the negative controls respectively. The experiment was set up in a completely randomized design. Data was collected on zone of growth inhibition, number of days to total tissue maceration and %weight loss of potato chips due to tissue maceration. The three concentrations of T. minuta (40%, 30% and 20%) recorded zones of inhibition of 7.167mm, 6.667mm and 6.1mm; and streptomycin sulphate recorded 8.83mm which were significantly different from C. frutescens and distilled water that recorded 0.00mm. In the potato chips assay, T. minuta and streptomycin sulphate showed a significant difference in the number of days (9days) to total tissue maceration and % weight loss from C. frutescens and water that took only 5days for the potato chips to be totally macerated. It can be concluded that extracts from T. minuta have antibacterial activity against Pectobacterium carotovorum.

關鍵字

In vitro ； growth inhibition ； Pectobacterium carotovorum ； Tagetes minuta ； Capsicum frutescens