This study aimed to understand the environmental adaption and visual preference of color of four interpretative signs in three different recreational environments. We used the focus group method to classified Penghu's landscape environments into three different categories, and also choose the color of interpretative signs. Photos depicting different recreational environment and colors were simulated by Adobe Photoshop used as a stimulus to assess environmental adaption and visual preference. 201 participants were asked to rate each photo for environmental adaption and their preference using a seven point Likert scale. The result showed that environment and signs color both affected the assessment of preference and adaptation of simulation images from the participants. The participants preferred the blue color interpretative sign than other colors in the city and country environment, and these four color interpretative sign were all not preferred in natural environments. The result also showed that the environmental adaption to interpretative sign could predict visual preference.