Ultrastructural Changes in a Cell Line from Plutella xylostella Treated with Bacillus thuringiensis Delta-endotoxin

小菜蛾細胞株受蘇力菌內毒素處理精微構造之改變

史惟馨(Wei-Shing Shih) ； 侯豐男(Roger F. Hou)

《中華昆蟲》 14卷4期 (1994/12) Pp. v+471-481

https://doi.org/10.6660/TESFE.1994042

摘要

Ultrastructural changes in a cell line, PX-1187, established from the diamondback moth, Plutella xylostella, treated with delta-endotoixn from Bacillus thuringiensis subsp. kurstaki (Btk) were observed using transmission electron microscopy. The normal PX-1187 cell has a large nucleus, several vesicles, intact mitochondria, Golgi complex and other organelles. When the cell were treated with delta-endotoxin for 30 min, the granules on mitochondrial cristae became separated from the inner membranes. The Golgi complex showed vacuoles and loose dictyosomes. After treating for 60 min, the cytoplasm was slightly vacuous, the mitochondrial cristae leaked, the dictyosomes showed damage and the cisternae were fused. After 90 min, parts of the cell membranes were perforated, the inner memberanes of the mitochondria lysed, and parts of the outer membranes disintegraterd. In addition, fusion of cisternae occurred and flocculent materials were seen in the Golgi complex. After 120 min, most cell membranes were destroyed, with the outer membranes of the mitochondria largely disintegrated. The Golgi complex exhibited lesions on its dictyosomes and fusion of flocculent materials. Cell lysis occurred by 150 min after treatment with delta-endotoxin.

關鍵字

Bacillus thuringiensis ； Plutella xylostella ； Diamondback moth ； delta-endotoxin ； PX-1187 cell line

並列摘要

由小菜蛾（Plutella xylostella）所建立之PX-1187細胞株經蘇力菌（Bacillus thuringiensis subsp. kurstaki, Btk）產生的δ-內毒素處理後，引起精微構造之改變，以穿透式電子影微鏡觀察。正常的PX-1187細胞具型大之細胞核、囊狀物數個、完整的粒線體、高基氏體及其它胞器。以δ-內毒素處理30分鐘後，粒線體內膜上之小顆粒有脫落的跡象；高基氏體四周形成一囊胞，膜狀物質較為鬆散，分泌物顆粒變小。處理60分鐘後，細胞質內空隙逐漸擴張，粒線體內膜已有破裂痕跡；高基氏體之膜狀物更疏鬆，分泌物顆粒數量減少。處理90分鐘後，部分細胞膜已有穿孔現象，粒線體內膜明顯破壞；高基氏體之膜狀物質已疏散斷裂，殘存少數分泌物顆粒；囊胞內模糊；細胞質內空隙繼續擴張。處理120分鐘，細胞膜大部分破壞，外膜崩解；粒線體外膜破裂，內膜已不存在；高基氏體之囊胞變大，只見不完整膜狀物質；細胞質內空隙變的更大。處理150分鐘後，細胞發生崩解。

並列關鍵字

蘇力菌 ； 小菜蛾 ； 內毒素 ； PX-1187細胞株