The honeybee mite Varroa jacobsoni Oudeman is the most serious pest of the honeybee in Taiwan. This study evaluates the possibility of utilizing a formic acid board to control this mite. Placing the formic acid board on the top of the comb frames produced the most effective results. There were no significant differences in mite control between application of 6ml/day and 8ml/day of formic acid. Both dosages gave more than an 80% control rate of Varroa mites. There were no significant differences in the survival rates of adult bees among different dosage treatments of formic acid from 2ml/day to 8ml/day. But treatments of 6ml/day and 8ml/day decreased capped worker cells an average of 15% and 58.5%, respectively. These treatments also influenced the amount of feeding by the bees on sugar syrup and pollen cake, but the influence of the 6ml/day treatment was less than the 8ml/day treatment. Application of 6ml/day of formic acid to control Varroa jacobsoni in a colony is recommended, and at this dosage adverse effects can be neglected.