自動化生物組織3D列印技術

Automated Skin Tissue 3D Bio-Printing Technology

李昌周 ； 林央正 ； 林東穎 ； 徐新怡 ； 駱婉珣 ； 廖智菁 ； 王羽淇

《機械工業雜誌》 467期 (2022/02) Pp. 74-82

摘要

歐盟2013年化粧品實施動物試驗禁令，臺灣也在2016年立法禁止。工研院研發自動化生物組織3D列印設備應用於全皮層皮膚組織，提供精準的材料與細胞布置能力及列印環境的控制，並保持細胞之活性及克服各列印階段所存在的培養與分化問題，成功建構出自動化3D列印之皮膚組織，並完成產品功能驗證及動物實驗。未來可作為化粧品、保養品的測試材料，亦可加入幹細胞發展成燒燙傷人工皮，以3D列印大量生產。

關鍵字

積層製造 ； 生物列印 ； 皮膚組織

並列摘要

In 2013, the EU announced ban on animal testing for cosmetics, and Taiwan as a member of global society also announced legislation to ban the aforesaid testing in 2016. ITRI develops automated 3D bio-printing equipment, which can be applied to produce full-thickness skin tissues, with capabilities to provide precise material and cell layout; precise printing environment control; maintain cell viability; overcome the cultivation and differentiation problems during each printing stage. By using this technology, we can successfully complete the skin tissue automated 3D printing, function verification, and animal experiments of the product. This technology can be used as testing material for cosmetics and skin-care products in the future; it can also be added with stem cells to develop the artificial skin for burned wounds and massive production with 3D printing to provide industrial benefits.

並列關鍵字

Additive manufacturing ； Bio printing ； Skin tissue

參考文獻

S. V. Murphy and A. Atala, “3D bioprinting of tissues and organs,” Nat. Biotechnol. 32(8), 773-785, 2014.

A. K. Nguyen, S. D. Gittard, A. Koroleva, S. Schlie, A. Gaidukeviciute, B. N. Chichkov, and R. J. Narayan, “Two-photon polymerization of polyethylene glycol diacrylate scaffolds with riboflavin and triethanolamine used as a watersoluble photoinitiator,” Regen. Med. 8(6), 725-738, 2013.

J. A. Inzana, D. Olvera, S. M. Fuller, J. P. Kelly, O. A. Graeve, E. M. Schwarz, S. L. Kates, and H. A. Awad, “3D printing of composite calcium phosphate and collagen scaffolds for bone regeneration,” Biomaterials 35, 4026-4034, Apr 2014.

D. B. Kolesky, R. L. Truby, A. S. Gladman, T. A. Busbee, K. A. Homan, and J. A. Lewis, “3D Bioprinting of Vascularized, Heterogeneous Cell-Laden Tissue Constructs,” Adv. Mater. 26(19), 3124–3130, 2014

L. A. Hockaday, K. H. Kang, N. W. Colangelo, P. Y. Cheung, B. Duan, E. Malone, J. Wu, L. N. Girardi, L. J. Bonassar, H. Lipson, C. C. Chu, and J. T. Butcher, “Rapid 3D printing of anatomically accurate and mechanically heterogeneous aortic valve hydrogel scaffolds,” Biofabrication 4(3), 035005, September 2012.