檜木精油對人類皮膚角質細胞增殖的影響及其抗氧化活性

檜木精油（Cypress essential oil）被用於芳香療法，研究指出其具有抑菌、抗真菌活性、抑制發炎反應、抗焦慮及緩解壓力等功效。然而，經由皮膚或呼吸器官進入人體的功能性與安全性是需要被重視的。因此，在本研究中，我們從市面上選購三種檜木精油，包括紅檜精油（Meniki essential oil; Chamaecyparis formosensis）、台灣扁柏精油（Taiwan Hinoki cypress essential oil; Chamaecyparis obtuse var. formosana）及台灣檜木精油（Taiwan cypress essential oil）探討其抗氧化活性，並測試其對於人類皮膚角質細胞株（Human skin keratinocyte cell line）HaCaT之細胞存活率。結果發現三種檜木精油對於清除1,1-diphenyl-2-picrylhydrazyl（DPPH）自由基能力，以紅檜及台灣扁柏精油的效果較好，螯合亞鐵離子能力則以台灣扁柏精油最好。三種檜木精油的抗氧化能力與酚類化合物總濃度呈現正相關。在細胞存活率的測試方面，紅檜及台灣扁柏精油濃度高於0.005％，台灣檜木精油則濃度高於0.025％時，會顯著抑制人類皮膚角質細胞增殖。因此，檜木精油使用於皮膚上宜小心謹慎。

關鍵字

紅檜精油；台灣扁柏精油；台灣檜木精油；抗氧化能力；細胞存活率

並列摘要

The cypress essential oil is used in aromatherapy, and studies have pointed out that it has anti-bacterial, anti-fungal, anti-inflammatory, anxiolytic-like, and anti-mitigation effects. However, the functionality and safety remain to be determined when the oil is administered through skin or the respiratory tract. Herein, we used human skin keratinocyte cell line (HaCaT) to explore the antioxidant activity and cytotoxicity of three kinds of commercial cypress essential oil including Meniki essential oil (Chamaecyparis formosensis), Taiwan Hinoki cypress essential oil (Chamaecyparis obtuse var. formosana), and Taiwan cypress essential oil. The results showed that the ability of scavenging 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radicals was superior with Meniki essential oil and Taiwan Hinoki cypress essential oil, while the ability of chelating ferrous ions was the best with the Taiwan Hinoki cypress essential oil. The antioxidant capacity of all three kinds of cypress essential oil was positively correlated with the total concentration of phenolic compounds. The concentrations required to significantly inhibit the proliferation of HaCaT were ＞0.005 % for Meniki essential oil and Taiwan Hinoki cypress essential oil and ＞ 0.025 % for Taiwan cypress essential oil. These results suggest that caution must be taken for the use of cypress essential oil on the skin.

並列關鍵字

Meniki essential oil ； Taiwan Hinoki cypress essential oil ； Taiwan cypress essential oil ； antioxidant capacity ； cell viability

參考文獻

Costa C, Tsatsakis A, Mamoulakis C, Teodoro M, Briguglio G, Caruso E, Tsoukalas D, Margina D, Dardiotis E, Kouretas D and Fenga C. (2017). Current evidence on the effect of dietary polyphenols intake on chronic diseases. Food Chem Toxicol. pii: S0278-6915(17)30621-X. doi: 10.1016/j.fct.2017.10.023.

Lee SH, Do HS and Min KJ. (2015). Effects of Essential Oil from Hinoki Cypress, Chamaecyparis obtusa, on Physiology and Behavior of Flies. PLoS One. doi: 10.1371/journal.pone.0143450.

陳秋萍 (2003)：施氮磷肥對紅檜及台灣扁柏苗木生長及養分積聚之影響。台北：國立臺灣大學森林學研究所碩士論文。