超臨界流體萃取薑黃對人類皮膚角質細胞的影響

薑黃（Curcuma longa, CL）具有抗氧化、抑制發炎反應、抗腫瘤、抑菌、抗真菌活性等生物活性。薑黃精油（turmeric essential oil）也被用於芳香療法，利用植物精油以增進健康的自然療法，則是透過接觸皮膚或呼吸器官進入人體，因此，本研究中除了探討其功能性外，也對於其安全性進行評估。本研究利用超臨界流體技術單獨使用二氧化碳（Carbon dioxide, CD）與加入共溶劑（Co-solvents, CS）提取出薑黃萃取物CL-CD與CL-CS，分析薑黃萃取物對人類皮膚角質細胞株（Human skin keratinocyte cell line, HaCaT）之細胞存活率，並利用彗星分析薑黃萃取物對於過氧化氫（Hydrogen peroxide, H_2O_2）誘發細胞氧化傷害的保護作用。研究結果發現在細胞存活率測試上，10－100μg/mL薑黃萃取物CL-CD及10－30μg/mL薑黃萃取物CL-CS，均不會影響HaCaT細胞生長。另外，薑黃萃取物CL-CD與CL-CS均能夠顯著抑制H_2O_2所誘發人類正常角質細胞（HaCaT）DNA傷害，且薑黃萃取物CL-CS抑制效果優於CL-CD。

關鍵字

薑黃；角質細胞；細胞存活率；彗星分析

並列摘要

Turmeric (Curcuma longa L.) is used in aromatherapy, and studies have pointed out that it has antioxidant, anti-inflammatory, anti-tumor properties, anti-bacterial, anti-fungal effects. However, the safety remains to be determined when the oil is administered through skin or the respiratory tract. In this study, Curcuma longa was extracted with CL-CD and CL-CS by supercritical carbon dioxide. Reactive oxygen species (ROS) such as free radicals are constantly generated in vivo and DNA is the most important target of oxidative stress. The comet analysis applied to DNA repair can address topics such as repair deficiency in disease or aging, modulation of repair by diet factors, and the effect of polymorphisms in repair genes. The results showed that the concentrations required to no inhibit the proliferation of HaCaT were 10-100 μg/mL CL-CD and 10-30 μg/mL CL-CS. The study was to evaluate the effect of Curcuma longa extracts on human keratinocyte cells by comet assay and to find out which properties contribute to this effect. Moreover, the CL-CS had a more protective effect than CL-CD. The study was to evaluate the effect of Curcuma longa extracts on human keratinocyte cells by comet assay and to find out which properties contribute to this effect. Finally, we will confer the Curcuma longa essential oils ratio that influences the skin care and cosmetic.

並列關鍵字

Curcuma longa ； Keratinocyte Cells ； Cell viability ； Comet Assay

參考文獻

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超臨界流體萃取薑黃對人類皮膚角質細胞的影響

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