- 期刊
- OpenAccess
Induction of Nrf2-dependent Antioxidation and Protection Against Carbon Tetrachloride-induced Liver Damage by Andrographis Herba (穿心蓮 Chuān xīn lián) Ethanolic Extract
摘要
Andrographis paniculata is a traditional Chinese herb and displays diverse biological activities including antioxidation, anti-tumorigenesis, anti-virus, and anti-atherogenesis. In this study, we investigated the up-regulation of ethanolic extract of A. paniculata (APE) on the antioxidant defense in rat livers and whether this enhancement protected against carbon tetrachloride (CCl_4)-induced liver damage. Male Sprague-Dawley rats were orally administered (i.g.) 0, 0.75, or 2 g/kg/d APE for 5 d. At d 6, rats were sacrificed and liver tissues were removed. Some animals (n=8) were intraperitoneally injected CCl_4 (1 mL/kg, 50% in olive oil) and blood was drawn 24 h after CCl_4 treatment. The results showed that APE increased hepatic glutathione (GSH) content and superoxide dismutase, GSH peroxidase, and GSH S-transferase activities in a dose-dependent manner (p ＜ 0.05). Results of immunoblotting and RT-PCR revealed that rats treated with APE had higher glutamate cysteine ligase catalytic and modifier subunits, heme oxygenase 1, superoxide dismutase 1, and GSH S-transferase Ya and Yb protein and mRNA expression than those of control rats. Moreover, APE increased Nrf2 nuclear translocation and Nrf2 binding to DNA in rat liver. In the presence of CCl_4, APE decreased hepatic thiobarbituric acid-reactive substances production and plasma aspartate aminotransferase and alanine aminotransferase activities. These results suggest that APE protection against CCl_4 insult is attributed, at least in part, to its up-regulation of antioxidant defense in rat liver.
延伸閱讀
- Li, F. Y., Chen, C. L., Wang, S. F., & Yang, J. L. (2018). 【論文摘要】The Effect of Edema Control in Patients With Breast Cancer- Comparison of Manual Lymph Drainage, Self-Massage and Epidermis Fascia Taping With Ultrasonography. 物理治療, 43(2), 169-170. https://doi.org/10.6215/FJPT.2018.75.P38
- Lin, L., & Chen, Y. H. (2022). 【論文摘要】Development of Spodoptera frugiperda (Lepidoptera: Noctuidae) on Three Different Artificial Diets and Controlling Effect with Chemical-free Pesticides. 花蓮區農業改良場研究彙報英文摘要版, (), 89-89. https://www.airitilibrary.com/Article/Detail?DocID=P20150127001-N202301050003-00006
- Hsu, B. Y., Kuo, Y. C., & Chen, B. H. (2014). Polysaccharide Isolated from Zizyphus jujuba (紅棗 Hóng Zǎo) Inhibits Interleukin-2 Production in Jurkat T Cells. Journal of Traditional and Complementary Medicine, 4(2), 132-135. https://doi.org/10.4103/2225-4110.124360
- 蔡美蘭（2012）。主題一：Functional regulation of Alu element of ACE gene in neuron cells主題二：The neuroprotection of Hu Yi-Neng in neuron- molecular analysis platform of natural products and diet supplements for their protective effects on neurodegenerative diseases〔碩士論文，高雄醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0011-2908201215570100
- Lin, L. T., Wu, S. J., & Lin, C. C. (2013). The Anticancer Properties and Apoptosis-inducing Mechanisms of Cinnamaldehyde and the Herbal Prescription Huang-Lian-Jie-Du-Tang (黃連解毒湯 Huáng Lián Jiě Dú Tang) in Human Hepatoma Cells. Journal of Traditional and Complementary Medicine, 3(4), 227-233. https://doi.org/10.4103/2225-4110.119732