Anticancer activity of Aristolochia ringens Vahl. (Aristolochiaceae)

Abidemi James Akindele ； Zahoor Wani ； Girish Mahajan ； Sadhana Sharma ； Flora Ruth Aigbe ； Naresh Satti ； Olufunmilayo Olaide Adeyemi ； Dilip Manikrao Mondhe

《Journal of Traditional and Complementary Medicine》 5卷1期 (2015/01) Pp. 35-41

https://doi.org/10.1016/j.jtcme.2014.05.001

摘要

Cancer is a leading cause of death worldwide and sustained focus is on the discovery and development of newer and better tolerated anticancer drugs especially from plants. The sulforhodamine B (SRB) in vitro cytotoxicity assay, sarcoma-180 (S-180) ascites and solid tumor, and L1210 lymphoid leukemia in vivo models were used to investigate the anticancer activity of root extracts of Aristolochia ringens Vahl. (Aristolochiaceae; 馬兜鈴mǎ dōu líng). AR-A001 (IC_(50) values of 20 μg/mL, 22 μg/mL, 3 μg/mL, and 24 μg/mL for A549, HCT-116, PC3, and THP-1 cell lines, respectively), and AR-A004 (IC_(50) values of 26 μg/mL, 19.5 μg/mL, 12 μg/mL, 28 μg/mL, 30 μg/mL, and 22 μg/mL for A549, HCT-116, PC3, A431, HeLa, and THP-1, respectively), were observed to be significantly active in vitro. Potency was highest with AR-A001 and AR-A004 for PC3 with IC_(50)values of 3 μg/mL and 12 μg/mL, respectively. AR-A001 and AR-A004 produced significant (p ＜ 0.05-0.001) dose-dependent inhibition of tumor growth in the S-180 ascites model with peak effects produced at the highest dose of 120mg/kg. Inhibition values were 79.51% and 89.98% for AR-A001 and AR-A004, respectively. In the S-180 solid tumor model, the inhibition of tumor growth was 29.45% and 50.50% for AR-A001 (120 mg/kg) and AR-A004 (110 mg/kg), respectively, compared to 50.18% for 5-fluorouracil (5-FU; 20 mg/kg). AR-A001 and AR-A004 were also significantly active in the leukemia model with 211.11% and 155.56% increase in mean survival time (MST) compared to a value of 211.11% for 5-FU. In conclusion, the ethanolic (AR-A001) and dichloromethane: methanol (AR-A004) root extracts of AR possess significant anticancer activities in vitro and in vivo.

關鍵字

Anticancer activity ； Aristolochia ringens ； Cytotoxicity ； Lymphoid leukemia ； Solid tumor

參考文獻

Aigbe FR, Adeyemi OO. The aqueous root extract of Aristolochia ringens (Vahl.) prevents chemically induced inflammation. Planta Med. 2011;77:PF28. http://dx.doi.org/10.1055/s-0031-1282416

Chhajed M, Sowle A, Baraskar R, Parihar N. Buserelin as a wonder drug for prostate cancer: a comprehensive study. Int J Pharm Res Sci. 2012;1:150-172.

Rao MRP, Adagale UR, Shetty A, Namjoshi P, Gaitonde P, Jain P. Cancer Immunotherapy; 2007. Accessed on 20th July, 2013, http://www.pharmainfo.net/reviews/cancer-immunotherapy

Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011;61:69-90.

Sikora K, Advani S, Koroltchouk V, et al. Essential drugs for cancer therapy: a World Health Organization consultation. Ann Oncol. 1999;10:385-390.