Evaluation of pancreatic regeneration activity of Tephrosia purpurea leaves in rats with streptozotocin-induced diabetes

Sumit K. Arora ； Prashant R. Verma ； Prakash R. Itankar ； Satyendra K. Prasad ； Kartik T. Nakhate

《Journal of Traditional and Complementary Medicine》 11卷5期 (2021/09) Pp. 435-445

https://doi.org/10.1016/j.jtcme.2021.03.001

摘要

Background and aim: Flavonoid rich plant Tephrosia purpurea (T. purpurea), commonly known as Sarpunkha has been used in traditional systems of medicine to treat diabetes mellitus. However, its effectiveness in promoting regeneration of pancreas in diabetes has not been investigated. Therefore, the present study was undertaken to evaluate pancreatic β-cells regeneration, antioxidant and antihyperlipidemic potentials of T. purpurea leaves extract, its fractions and main constituent Rutin in diabetic rats. Experimental procedure: The leaves extract and its fractions were first screened for acute and sub-chronic antidiabetic activity in a dose range of 250-500 mg/kg orally. Further, fractions with potent antidiabetic activity were screened for pancreatic β-cells regeneration activity using histopathological studies and morphometric analysis, which was followed by estimation of biochemical parameters. Results and conclusion: The most significant antidiabetic, pancreatic regeneration and antihyperlipidemic activity was exhibited by n-butanol soluble fraction of ethanol extract at the dose level of 500 mg/kg. Histopathology revealed that treatment with this fraction improved the β-cell granulation of islets and prevented the β-cells damage which was further confirmed by morphometric analysis. Thus, the present study validated the traditional use of T. purpurea plant in the treatment of diabetes, which might be attributed to pancreatic β-cells regeneration potential of its active constituent Rutin. Taxonomy (classification by EVISE): Traditional Medicine; Metabolic Disorder; Experimental Design; Cell Regeneration and Histopathology.

關鍵字

Pancreatic β-cell ； Diabetes mellitus ； Plant extracts ； Polyphenol ； Antioxidant activity ； Biochemical estimation

參考文獻

Choudhury H, Pandey M, Hua CK, et al. An update on natural compounds in the remedy of diabetes mellitus: a systematic review. J Tradit Complement Med. 2018;8(3):361-376. https://doi.org/10.1016/j.jtcme.2017.08.012

Lartey NL, Asare-Anane H, Ofori EK, et al. Antidiabetic activity of aqueous stem bark extract of Annickia polycarpa in alloxan-induced diabetic mice. J Tradit Complement Med. 2020. https://doi.org/10.1016/j.jtcme.2020.02.001

Jani DK, Goswami S. Antidiabetic activity of Cassia angustifolia Vahl. and Raphanus sativus Linn. leaf extracts. J Tradit Complement Med. 2020;10(2): 124-131. https://doi.org/10.1016/j.jtcme.2019.03.002

Skyler JS, Bakris GL, Bonifacio E, et al. Differentiation of diabetes by pathophysiology, natural history, and prognosis. Diabetes. 2017;66(2):241-255. https://doi.org/10.2337/db16-0806

Robertson R, Harmon J. Diabetes, glucose toxicity, and oxidative stress: a case of double jeopardy for the pancreatic islet beta cell. Free Radic Biol Med. 2006;41:177-184. https://doi.org/10.1016/j.freeradbiomed.2005.04.030