At present, China is making efforts to build Guangdong, Hong Kong, Macao and Dawan District and upgrading the regional economic structure. Guangzhou, which is located in the center of the Pearl River Delta, is the most prosperous trading place in the international metropolis and South China, and the structure of Guangzhou Port Collection and Transportation is optimal. Affects whether Guangzhou Port can meet the increasing volume of freight. This paper focuses on the status quo of Guangzhou Port Collection and Distributing System, compares the hardware, software and development characteristics of the three modes of transportation in Guangzhou Port, and makes a linear regression forecast for the future volume of cargo throughput in Guangzhou Port. The multi-level factor analysis method is used to evaluate the three collection and distribution modes of highways, railways and waterways commonly used in Guangzhou Port Collection and Transportation, so as to find out the main problems of the Guangzhou Port Collection and Distributing System. Based on this, Suggestions for the optimization of the Guangzhou Port Collection and Distributing System.