The laboratory is the ability to cultivate students' research and innovation, conduct quality education, and develop the cradle of high ‐quality talents. However, the laboratory has the characteristics of sudden and exploring, leading to frequent laboratory accidents, bringing huge losses and adverse effects to schools and society. The safety of university laboratories has become a topic that people have paid attention to in recent years, and the safety management of laboratory needs to be strengthened. In order to improve and improve the safety emergency management of laboratory, this article starts from the importance of laboratory management and construction, and is targeted at the four aspects that affect laboratory security: basic safety management, personnel safety management, operation safety management, chemical safety management, design survey Questionnaire and proposed assumptions, analyze the data of the obtained data with SPSS, use AMOS software to build a structural equation model, perform factors analysis and path analysis, and verify the assumptions proposed to better improve laboratory security provision Targeted and feasible suggestions. This article uses SPSS and AMOS software to analyze, verify the assumptions, and draw the following conclusions: Laboratory rules and regulations and laboratory management have positive impacts, and the effect is the most significant.