- 期刊
培養液中添加生長素和血紅素對體外成熟與體外培養牛卵母細胞發育能力之影響
Effects of growth hormone and hemoglobin supplementation on the developmental competence of bovine oocytes during in vitro maturation and culture
摘要
本試驗旨在探討牛卵母細胞於體外成熟過程中添加不同濃度之豬生長素，及體外培養過程中添加不同濃度之牛血紅素，對牛卵母細胞發育能力之影響，以提昇體外生產系統之效率。自屠宰場取得乳牛與黃牛卵巢攜回試驗室後， 立即抽取卵丘─卵母細胞複合體（cumulus-oocyte complex；COC）進行試驗。結果發現，添加豬生長素0.01、0.1 與1μg/ml對牛卵母細胞之卵裂率分別為56.0%、55.1%與51.9%，均顯著高於對照組之43.3%（P ＜ 0.05）。添加牛血紅素0.01與0.1μg/ml組發育至桑椹胚與囊胚之百分率分別為16.7%與15.2 %，顯著高於對照組之12.1%（P ＜ 0.05）；而添加牛血紅素1μg/ml組發育至桑椹胚與囊胚之百分率為8.2%，則顯著低於對照組（P ＜ 0.05）。試驗結果顯示，牛卵母細胞體外成熟時，於培養液中添加豬生長素0.01、0.1 與1μg/ml，可促進其卵裂率，而於體外受精後之培養過程中，添加牛血紅素0.01與0.1μg/ml則有促進牛胚發育至桑椹胚與囊胚之能力。本試驗之結果將可供牛卵母細胞體外成熟培養之參考。
並列摘要
This experiment was conducted to investigate the effects of growth hormone and hemoglobin supplementation on the developmental competence of bovine oocytes during in vitro maturation and culture, and efficiency promotion in bovine embryos in an in vitro production system. The results show that the cleavage rates were 56.0 %, 55.1 % and 51.9 % when the maturation medium was supplemented with porcine growth hormone 0.01, 0.1 and 1μg/ml, respectively. These rates were significantly higher than the control 43.3% (P ＜ 0.05). The percentage of embryos developed to morula plus blastocyst were 16.7% and 15.2% when the culture medium was supplemented with bovine hemoglobin 0.01 and 0.1μg/ml, respectively. These results were significantly higher than the control 12.1% (P ＜ 0.05). However, the percentage of embryos developed to morula plus blastocyst when supplemented with hemoglobin 1 μg/ml was significantly lower than the control (8.2% vs. 12.1%) (P ＜ 0.05). These results indicated that porcine growth hormone supplementation at 0.01, 0.1 and 1μg/ml could improve the bovine oocyte cleavage rate during in vitro maturation. Supplementation of bovine hemoglobin 0.01 and 0.1μg/ml could improve the developmental competence of bovine embryos during in vitro culture. This study provides optimal application for future bovine in vitro production systems.
延伸閱讀
- 林育安、嚴為怜、許芳諭、曾翊愷、謝松桓、廖明輝、陳銘正（2009）。豬濾泡液與賀爾蒙對卵母細胞體外成熟、激活暨發育之影響。弘光學報，(56)，75-84。https://doi.org/10.6615/HAR.200908.56.07
- 林怡慧（2004）。成人周邊血液造血幹/前驅細胞在紅血球生成過程中之分化特性研究〔碩士論文，臺北醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0007-1704200714562414
- 游軒銘（2015）。飼料添加富硒裂褶菌醱酵物在F4ac型大腸桿菌灌注下對離乳仔豬生長性能與免疫反應之影響〔碩士論文，國立宜蘭大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0046-2007201519354200
- Boparai, R. K., Arum, O., Miquet, J. G., Masternak, M. M., Bartke, A., & Khardori, R. K. (2015). Resistance to the Beneficial Metabolic Effects and Hepatic Antioxidant Defense Actions of Fibroblast Growth Factor 21 Treatment in Growth Hormone-Overexpressing Transgenic Mice. International Journal of Endocrinology, 2015(), 503-513-057. https://doi.org/10.1155/2015/282375
- Chen, W. Y., Yang, J. G., Huang, S. H., & Li, P. H. S. (1998). Effects of Cyclophosphamide on Maturation and Subsequent Fertilizing Capacity of Pig Oocytes in Vitro. The Chinese Journal of Physiology, 41(2), 75-83. https://www.airitilibrary.com/Article/Detail?DocID=03044920-199806-41-2-75-83-a