狼尾草營養成分近紅外光分析檢量線之探討

本研究目的為建立狼尾草品質之近紅外光分析檢量線，測定成分包括：粗蛋白質（CP）、酸洗纖維（ADF）、中洗纖維（NDF）、及水溶性碳水化合物（WSC）。首先掃描不同地點之割期試驗樣品332個，選擇其中具代表性樣品119個建立基礎檢量線，再以畜牧場廢水灌施試驗樣品36個及青貯樣品48個進行擴充。廢水灌施試驗樣品光譜之整體距離（Global H）均在3.0之內，鄰接距離（Neighborhood H）介於0.6-1.5之間，與基礎檢量線樣品光譜特性差別不大；而青貯樣品之整體距離均在5.0以上，顯然青貯後植體之光譜特性有較大的改變。更新後CP、NDF、ADF、WSC檢量線之決定係數（R^2）分別為0.99、0.95、0.98、0.99，檢量線標準機差（SEC）分別為0.45%、1.17%、0.80%、0.45%，交互驗證標準機差（SECV）分別為0.59%、1.37%、1.03%與0.59%，而樣品實測值標準偏差與驗證標準機差之比值（RPD）分別為7.8、3.9、4.8、8.8，均高於可接受之標準，且準確度高於先前建立之盤固草檢量線。本研究除一般品質成分之評估外，尚提供對植體WSC含量的快速分析，供青貯調製之參考。

關鍵字

快速測定；粗蛋白質；中洗纖維；酸洗纖維；水溶性碳水化合物；青貯草

並列摘要

The purpose of this research was to set up the calibration equations for near infrared spectrum (NIRS) for rapid crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), and water-soluble carbohydrates (WSC) measurement analysis in napiergrass. Three hundred thirty-three samples from different cutting interval trials at different locations were scanned using NIRS. One hundred nineteen samples were selected to set up the basic calibration equations. The basic calibration equations were rebuilt by adding 36 samples from a livestock wastewater application trial and 48 samples from a silage making trial. The global H distance was lower than 3.0 and the neighborhood H was between 0.6 and 1.5 for all samples from the wastewater trial. This indicated that they were similar to the basic calibration samples in spectrum characteristics. The global H values for the silage samples were higher than 5.0, indicating that the spectrum characteristics had significantly changed after ensiling. The R2 of the updated regressions for CP, NDF, ADF, and WSC were 0.99, 0.95, 0.98, and 0.99, respectively. The SEC were 0.45%, 1.17%, 0.80%, and 0.45%, and the SECV (standard error of cross verification) were 0.59%, 1.37%, 1.03%, and 0.59%, respectively. The RPD (ratio of the constituent standard deviation to the standard error of prediction) were 7.8, 3.9, 4.8, and 8.8, indicating that the four calibration equations were acceptable for predicting the component accurately. The accuracy was higher than that for pangolagrass set up previously. Besides determining the nutritional components, this method was also applied to determine the WSC content, which is an useful information for ensiling forages.

並列關鍵字

Rapid analysis ； Crude protein ； Acid detergent fiber ； Neutral detergent fiber ； Water soluble carbohydrates ； Silage

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