CPPU對盤固草A254未成熟花穗誘導癒合組織形成及植株再生之影響

本試驗以盤固草A254（Digitaria decumbens）未成熟花穗為培殖體，接種於含有2, 4-D（2, 4-dichlorophenoxyacetic acid）及CPPU（N-（2-chloro-4-pyridyl）–N'-phenylurea）的MS（Murashige and Skoog）培養基，探討CPPU對盤固草A254癒合組織形態及癒合組織誘導率之影響。在含有2,4-D 2.0 mg/l的MS培養基，隨著CPPU濃度由0.0 mg/l增加至0.5 mg/l，白色緊密癒合組織的誘導率由13.4%增加至90.0%，CPPU顯著影響誘導的癒合組織形態。將不同型態的癒合組織，接種至含有TDZ（N-phenyl-N'-1,2,3-thiadiazol-5-yl urea）0.05 mg/l的MS培養基，其中2,4-D 2 mg/l及CPPU 0.1 mg/l誘導的白色緊密癒合組織有80.0%可分化成完整植株，而透明鬆軟的癒合組織甚少植株的分化。因此，CPPU與白色緊密癒合組織的形成具有密切的關係，而癒合組織的型態往往決定盤固草植株的再生能力，未來可應用此盤固草植株再生系統進行基因轉殖之研究。

關鍵字

植株再生；癒合組織形態；盤固草

並列摘要

A system for callus induction and plant regeneration of Pangolagrass A254 (Digitaria decumbens) was developed using immature inflorescences as explants. Explants were cultured in MS (Murashige and Skoog) medium supplemented with different concentration of 2,4-D (2, 4-dichlorophenoxyacetic acid) and CPPU (N-(2-chloro-4-pyridyl)-N'-phenylurea). The effects of CPPU on callus induction frequency and morphology were examined. When the concentration of CPPU was increased from 0.0 mg/l to 0.5 mg/l with 2,4-D 2.0 mg/l in MS medium, the frequency of white and compact callus induction was increased from 13.4 % to 90.0 %. CPPU significantly influenced the morphology of calli induced. Different types of calli were cultured in MS medium supplemented with TDZ (N-phenyl-N'-1, 2, 3-thiadiazol-5-yl urea) 0.05 mg/l. The percentage of plant regenerated from white and compact callus induced with 2,4-D 2.0 mg/l and CPPU 0.1 mg/l medium was as high as 80.0 %. There was little plant regeneration from friable and transparent callus. The frequency of plant regeneration depended on the type of callus that was affected by CPPU concentration. We concluded that CCPU was involved in the white and compact callus formation that is important for plant regeneration of Pangolagrass. We could use the plant regeneration system for gene transformation in the future.

並列關鍵字

Plant regeneration ； Callus type ； Pangolagrass

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