豬胰脂肪瓷相關蛋白質第1型之互補DNA選殖與分析

本試驗目的為選殖豬胰脂肪瓷相關蛋白質第1型之互補DNA（cDNA），並分析其核啟酸瓷與轉譯後胺基酸序列與人類和狗之間的同一性。試驗萃取自豬胰臟之mRNA經RT-PCR後，利用巢式聚合瓷連鎖反應（Nest PCR）方法增幅豬胰脂肪瓷相關蛋白質第1型之互補DNA，並利用Basic Local Alignment Search Tool（BLASTn），進行核啟酸序列與胺基酸序列之比對；結果顯示，此項選殖序列中與人類以及狗之胰脂肪瓷相關蛋白質第1型cDNA，其同一性（identities）均為87%。胺基酸序列比對方面，得知此項選殖序列中與人類以及狗之胰脂肪瓷相關蛋白質第1型，其同一性為85%。因此總合上述之結果，推論豬之胰脂肪瓷相關蛋白質第1型，應與人類以及狗者具有相同之蛋白質結構與來自共同之祖先。同時本試驗選殖豬胰脂肪瓷相關蛋白質第1型之cDNA，將可供後續利用生物反應器，表現胰脂肪瓷相關蛋白質第1型之重組蛋白質，藉以提升離乳仔豬脂肪之利用率。

關鍵字

豬胰脂肪瓷相關蛋白質第1型；巢式聚合瓷連鎖反應；同一性

並列摘要

The aim of this study was to clone cDNA of porcine pancreatic lipase-related protein 1. The mRNA was derived from porcine pancreas by RT-PCR, and the cDNA product of pancreatic lipase-related protein 1 was amplified by nested PCR. The nucleotide sequences and amino acid residues of porcine pancreatic lipase-related protein 1 were aligned by the Basic Alignment Search Tool (Blast). The results showed that the identity of porcine cDNA of pancreatic lipase-related protein 1 was 87% either between swine and human or between swine and canine. Similar results were also found that the identities were 85%, while comparison of pancreatic lipase-related protein 1 of amino acid residues either between swine and human or between swine and canine. According to the very high identities (87% and 85%) in protein conformation of pancreatic lipase-related protein 1 between swine and human and canine, we could speculate that all of them stemmed from the common ancestor. Meanwhile, we will also transform the novel of porcine pancreatic lipase-related protein 1 of cDNA into the appropriate bioreactor to yield the recombinant pancreatic lipase-related protein 1 to improve fat digestion for postweaning piglets.

並列關鍵字

Porcine pancreatic lipase-related protein 1 ； Nested PCR ； Identity

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