山羊超級排卵最適條件之探討

本研究主要建立山羊有效超級排卵方式，試驗一評估雜交種與純種努比亞山羊的超級排卵反應，以雜交種母羊與純種母羊各12頭，分別進行超級排卵處理，在發情後第7日，採用腹中線剖腹術進行羊胚之沖出及回收，檢視其卵巢反應。試驗結果顯示相同超級排卵處理方式，所得平均黃體數、胚回收數及可用胚數等均以雜交種山羊顯著較純種努比亞母羊為高（P＜0.05）。試驗二探討在激性腺素（gonadotrophin）超級排卵處理之前列腺素（prostaglandin, PGF2α）注射時間，PGF2α在以激性腺激素超級排卵處理時分三組，使用純種努比亞母羊共36頭，第一、二及三天分別注射PGF2α後評估母羊卵巢之反應。試驗結果顯示在超級排卵處理之第三天注射PGF2α，平均黃體數，胚回收數及可用胚數，均顯著（P＜0.05）高於超級排卵處理之第一、二天注射者。

關鍵字

山羊；超級排卵；激性腺素

並列摘要

This study was conducted to investigate the essential factors affecting superovulation of goats in order to establish the superovulation methodology. In experiment I, the differences of superovulatory response were compared in Crossbred goats and Nubian goats. This experiment was conducted with twelve goats of each breed. Seven days after mating, the embryos were flushed and recovered through the aid of mid ventral laparotomy and ovarian response was recorded. The results showed that the number of corpora lutea/does, recovered embryos/does and viable embryos/does of crossbred does were significantly higher than those of Nubian does (P ＜ 0.05). In experiment Ⅱ, PGF2α were administered at different time after gonadotrophins injection for superovulation. Thirty-six Nubian goats were randomly divided into three equal groups. Ovarian responses were evaluated following PGF2α administrated at the time of the first, second or third day gonadotrophins injection for superovulation. The number of corpora lutea, recovered embryos and viable embryos of PGF2α treatment at third day group were significantly higher than those in the first or second day treatment group (P ＜ 0.05).

並列關鍵字

Goat ； Superovulation ； Gonadotrophin

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