狼尾草品系之ISSR親緣分析

本研究利用ISSR（inter simple sequence repeat）標記方法以探討狼尾草品種（系）間之遺傳親緣關係、分子指紋圖譜與品種鑑別，所用品系包含了狼尾草台畜草二號（cv.TLG2）、紫色狼尾草（NBM）及其雜交後裔、以及未知親本之狼尾草等29個品系材料。結果顯示受測品系平均遺傳相似性達0.45（45%），其中NG11、NG16、NG18、TLG2彼此間具高度親緣關係（GS ＞ 0.8），然品系NG01與NG04對於另一群品系NP03、NP04、NP05、NP07具較低程度的親緣關係（GS ＜ 0.2），而未知譜系之狼尾草經群集分析後，顯示其與cv.TLG2及NBM具有相當程度上之親緣關係。由參試品系之株高等外表性狀分群與ISSR親緣分群結果比對，亦能反應其實際遺傳親緣關係。於品種鑑別方面，以3組ISSR引子即可將目前之栽培品種狼尾草台畜草二號與其他品系區隔，將可作為品種鑑別之用。

關鍵字

狼尾草； ISSR標記；親緣關係

並列摘要

Twenty-nine napiergrass lines including cv.TLG2, strain brown mid-rib napiergrass (NBM), their hybrids and the unknown parent lines were used to determine the genetic relationship, molecular fingerprint and variety identification by ISSR (inter simple sequence repeat) marker in this study. The results showed that the average genetic similarity (GS) was 0.45 for the evaluated napiergrass germplasms by 65 ISSR markers. There was a higher GS (GS ＞ 0.8) within the group of hybrids, i.e., NG11, NG16, NG18 and cv.TLG2, but lines of NG01 and NG04 possessed the lowest GS along with a group of hybrids, i.e., NP03, NP04, NP05 and NP07 (GS ＜ 0.2). The genetic backgrounds of unknown parent lines were derived from the combination of cv.TLG2/ NBM after cluster analysis. The genetic relationship from morphological trait analysis showed that it was similar to the result determined by ISSR markers. Further, the results also showed that 3 sets of ISSR markers could help distinguishing cv.TLG2 from the rest of the evaluated napiergrass germplasms.

並列關鍵字

Napiergrass ； ISSR marker ； Genetic relationship

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