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不同蛋白質來源之人工乳對18日齡離乳仔豬消化道蛋白酶活性發展之影響

Effects of various protein sources in creep feed on digestive tract protein enzymes development of piglets weaned at 18 days of age

摘要


本試驗探討飼糧中主要蛋白質來源包括脫脂乳粉(dried skim milk, DSM)、濃縮馬鈴薯蛋白(potato protein concentrate, PPC)或濃縮大豆蛋白(soya protein concentrate, SPC)對18日齡離乳仔豬消化道蛋白酶活性之影響。各主要蛋白質來源佔人工乳中粗蛋白質總含量之三分之一。試驗採用18日齡之三品種〔Landrace×Yorkshire雜交母豬(♀)配Duroc公豬(♂)〕雜交仔豬45頭(來自6胎,其中4胎為9頭,另2胎合計9頭,以5胎計),每胎內之仔豬,依體重及性別平均分配至含DSM、PPC或SPC之飼糧處理組,每隻豬視為一試驗單位,各處理組均為一欄,每欄15頭,各處理組仔豬分別於離乳後第0、5及10天各處理組屠宰1頭,試驗為期10天。結果顯示,離乳後第5天,脫脂乳粉組者在消化道器官重量方面,在離乳後10天,其胃黏膜重量及離乳後5天及10天之胰臟組織重量,分別較濃縮馬鈴薯蛋白組者為重(P<0.05),且消化道中胃黏膜中蛋白酶活性、胰臟組織中胰蛋白酶及胰凝乳蛋白酶活性亦較高(P<0.05)。離乳後第10天,餵飼濃縮大豆蛋白組者與脫脂乳粉組者,除胰凝乳蛋白酶活性以脫脂乳粉組較高(P<0.05)外,其餘在消化道器官重量及消化道蛋白酶活性均無顯著差異存在。濃縮馬鈴薯蛋白組之各項測定指標較脫脂乳粉組者為低(P<0.05)。

並列摘要


The objective of this study was to investigate the effects of protein sources in creep feed on digestive tract protein enzymes development of piglets weaned at 18 days of age. The protein sources were dried skim milk (DSM), potato protein concentrate (PPC) or soya protein concentrate (SPC). The protein source provided one third of the total crude protein in the creep feed. Forty-five piglets of Landrace × Yorkshire (♀) crossed with Duroc(♂) from six litters weaned at 18 day-old were used. According to body weight and sex, the piglets were allotted into three pens, each with fifteen piglets. The pens regarded as experimental units were randomly allotted into three dietary treatments, which contained DSM, PPC or SPC as the main source of protein for ten days postweaning. All piglets were slaughtered at 0, 5 and 10 days, respectively. Fifteen piglets (selected from each litter of each pen) were offered to slaughter at each time. The results indicated that feeding with various protein sources in creep feed had a tremendous effect on the gastrointestinal development. At 5th days after weaning, piglets fed DSM had heavier weight of gastric mucosa and pancreas tissue than that of PPC and SPC treatments (P < 0.05). The DSM also showed a more activity of pepsin in gastric mucosa, and more activity of trypsin and chymotrypsin in pancreas tissue (P < 0.05). At 10 days after weaning, the gastrointestinal tract weight and protease activity did not differ significantly between SPC and DSM treatments, except the activity of chymotrypsin was greater in DSM (P < 0.05). Each determination in PPC treatment was less than that in DSM one (P < 0.05).

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