稀釋液種類對台灣水鹿精液冷藏後精液品質及懷孕率之影響

Effects of extenders on the quality and fertility of cooled Formosan sambar stag semen

王治華(Chih-Hua Wang) ； 林信宏(Hsin- Hung Lin) ； 康獻仁(Shann-Ren Kang) ； 郭卿雲(Ching-Yun Kuo) ； 宋文霖(Wen-Lin Song) ； 曾進輝(Chin-Hui Tseng) ； 鄭木榮(Mu-Jung Cheng) ； 劉炳燦(Bing-Tsan Liu) ； 沈朋志(Perng-Chih Shen)

《畜產研究》 43卷4期 (2010/12) Pp. 317-326

摘要

本試驗旨在探討稀釋液種類對台灣水鹿精液冷藏後精液品質及懷孕率之影響。結果顯示，利用電激採精法所得台灣水鹿精液之平均精子活力為4.7；平均精子濃度為8.9×10^8/mL；平均精子存活率為87.0%。以Tris-citric acid-yolk（TCY）及TES-yolk（TY）稀釋之公鹿精液於剛稀釋（0h）或冷藏12h後之精子活力均為5.0，而於citric acid-yolk（CY）稀釋液組則自5.0降至4.0；且上述3處理組於冷藏12h後之精子存活率（TCY：86.8vs.89.3%；TY：88.1vs.92.1%；CY：84.2vs.88.3%）及頭帽完整性（TCY：82.3vs.86.5%；TY：82.5vs.88.5%；CY：77.3vs.85.4%）均顯著低於剛稀釋時（0h）（P＜0.05）。將TCY、TY或CY稀釋液組所冷藏12h之台灣水鹿精液均分別進行人工授精後，母水鹿之懷孕率（TCY：70%；TY：80%；CY：60%）及分娩率（TCY：70%；TY：80%；CY：60%）於3種處理組間均無顯著差異（P＞0.05）。綜合前述結果，台灣水鹿之精液應用TY稀釋液進行冷藏可得較佳之精液品質，且應用三種稀釋液稀釋之後精液進行人工授精皆可成功產仔。

關鍵字

台灣水鹿 ； 精液冷藏保存 ； 稀釋液 ； 精液品質 ； 懷孕率

並列摘要

The aim of this study was to examine the effects of extenders on the quality and fertility of cooled Formosan sambar stag semen after artificial insemination. Result showed that the sperm motility score (SMS), sperm concentration, and sperm viability (VIAB) of Formosan sambar stag semen collected by electro ejaculation were 4.7, 8.9×108 sperms/mL and 87.0%, respectively. The SMS of semen diluted with TCY or TY extenders were not decreased by cooling at 4℃ for 12 h (5.0 and 5.0), but those of semen diluted with CY extender were decreased from 5.0 to 4.0 (P ＜ 0.05). The VIAB (TCY: 86.8 vs. 89.3%; TY: 88.1 vs. 92.1%; CY: 84.2 vs. 88.3%) and acrosomal integrity (NAR) (TCY: 82.3 vs. 86.5%; TY: 82.5 vs. 88.5%; CY: 77.3 vs. 85.4%) of the semen diluted with TCY, TY or CY extenders and cooled at 4℃ for 12 h were significantly lower (P ＜ 0.05) than those of semen freshly diluted with these extenders without cooling storage (0 h). The pregnant rates (TCY: 70%; TY: 80%; CY: 60%) and parturient rates (TCY: 70%; TY: 80%; CY: 60%) were similar among estrus synchronized Formosan sambar hinds after artificial insemination with the stag semen diluted with TCY, TY or CY extenders cooled at 4℃ for 12 h. In conclusion, Formosan sambar stag semen diluted with TCY or TY extenders had a better sperm quality. Despite of the extender used, the pregnant rate and the parturient rate after AI using the cooled semen were not affected.

並列關鍵字

Formosan sambar deer ； Semen cooling storage ； Extender ； Semen quality ； Pregnant rate