- 期刊
家禽屠宰場廢水處理活性污泥之細菌多樣性分析
Bacterial diversity of activated sludge treating slaughterhouse wastewater
摘要
本研究之目的在於探討不同污泥齡處理家禽屠宰場廢水活性污泥中之細菌多樣性，藉以瞭解活性污泥中細菌群落之變化情形。使用商業核酸萃取套組直接分別萃取7、14、21及28 d污泥齡之活性污泥樣品中之微生物DNA，其後以細菌16S核醣體核酸小單位基因專一性引子進行PCR增幅放大，並將增幅片段選殖於TA選殖套組，以建立16S rRNA基因庫，並進行DNA定序與細菌多樣性分析。由4種樣品所得幾乎完整的16S rRNA基因序列數目分別為116、122、119及108個，操作分類單位數目則分別為73、86、80及41個。結果顯示，不同污泥齡的樣品菌相分布有差異，而所屬菌門有Acidobacteria、Actinobacteria、Bacteroidetes、Firmicutes、Gemmatimonadetes、Chloroflexi、Candidatus Saccharibacteria、Planctomycetes、Proteobacteria、Verrucomicrobia 及unclassified bacteria，4種樣品中皆以Proteobacteria佔最多數（＞ 70%）。在株系操作分類單位百分比的分析中顯示，污泥齡28 d樣品的百分比最低（38.0%），表示活性污泥中的菌相組成漸趨單純，且以光合菌屬Rhodobacter最為豐富，COD的去除率可達96%且出流水之微生物濃度亦較低，僅8 mg VSS/L，期能將此結果應用於較大規模家禽屠宰場廢水之處理。
並列摘要
The objective of this study was to investigate the bacterial diversity of activated sludge with different sludge retention time (SRT) to treat slaughterhouse wastewater. The microbial DNA of activated sludge samples from those with SRTs of 7, 14, 21 and 28 days were extracted directly using a commercial kit and bead-beating protocol, respectively. The 16S ribosomal RNA genes of bacteria from 4 activated sludge samples were amplified with bacterial specific sets of primers by PCR. The amplicons were ligated into TA cloning vectors to construct 16S rRNA gene libraries for DNA sequencing and bacterial diversity analyses. A total of 312 almost full-length 16S rRNA gene clones from 4 activated sludge samples were obtained. The results indicated that the bacterial profiles comprising of Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, Gemmatimonadetes, Chloroflexi, Candidatus Saccharibacteria, Planctomycetes, Proteobacteria, Verrucomicrobia and unclassified bacteria differed in SRT samples. The most of these communities belonged to Proteobacteria phylum (＞ 70%). In OTU analysis, the OTU percentage of 28-day SRT sample was the lowest (38.0%) which revealed that the bacterial profile tended towards simplicity and photosynthetic bacterium Rhodobacter was the majority. However, the COD removal rate under SRT28 test run was still as high as 96% and the concentration of microorganisms in effluent waster was very low as 8 mg VSS/L. The study is expected to apply for the treatment of lager scale wastewater from poultry slaghterhouse.
延伸閱讀
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