青割玉米與大豆間植營養成分近紅外光分析檢量線之探討

Calibration equations for determining nutritional components of forage corn and soybean intercropping by near-infrared spectroscopy analysis

朱明宏(Ming-Hung Chu) ； 王紓愍(Shu-Min Wang) ； 陳嘉昇(Chia-Sheng Chen)

《畜產研究》 48卷4期 (2015/12) Pp. 297-303

摘要

本研究之目的為建立青割玉米與大豆間植營養成分近紅外光分析檢量線，測定之營養成分包含：粗蛋白質（crude protein, CP）、酸洗纖維（acid defergert fiber, ADF）及中洗纖維（neufral defergent fiber, NDF）。首先以376個不同來源之青割及青貯樣品進行基礎檢量線的建立，基礎檢量線之CP、ADF及NDF交互驗證標準偏差（SECV）分別為0.85、1.09及1.25%，檢量線標準偏差（SEC）分別為0.71、0.80及1.02%，決定係數（R^2）分別為0.89、0.92及0.94。基礎檢量線建立後另以52個青割及青貯樣品進行測試與更新。測試之結果，CP、ADF及NDF之平均偏差分別為-0.19、-0.50及0.13%，預測標準偏差（SEP）分別為0.89、1.49及1.66%，斜率（slope）分別為1.01、0.93及0.93，表示基礎檢量線對不同來源之樣品具備相當之測定準確度。其後以測試樣品進行檢量線之擴充，更新後檢量線CP、ADF及NDF之SECV分別為0.81、1.07及1.29%，SEC分別為0.64、0.83及1.08%，R^2分別為0.91、0.92及0.93，樣品實測標準偏差與驗證標準偏差之比值（RPD）分別為3.4、3.6及3.8，均達可接受之準確度。本檢量線之樣品歧異度大，所建立檢量線經測試結果已具備充分之準確度，可作為青割玉米與大豆間植的芻料品質快速測定。

關鍵字

青割玉米 ； 大豆 ； 近紅外光分析 ； 快速測定

並列摘要

The aim of this research was to establish the calibration equations for determining the chemical components of forage corn and soybean intercropping by near infrared spectroscopy (NIRS). The nutrient constituents included crude protein (CP), acid detergent fiber (ADF) and neutral detergent fiber (NDF) were quantified. The 376 samples were collected and scanned by NIR spectrums for setting up the basic calibrations. The standard error of cross verification (SECV) for CP, ADF and NDF were 0.85, 1.09 and 1.25%, the standard error of calibration (SEC) were 0.71, 0.80 and 1.02% and the regression coefficients (R^2) were 0.89, 0.92 and 0.94, respectively. The basic calibration equations were verified and updated with another 52 samples. The results showed that the biases of the means for CP, ADF and NDF were -0.19, -0.50 and 0.13%, the standard error of prediction (SEP) were 0.89, 1.49 and 1.66%, the slope were 1.01, 0.93 and 0.93, respectively. It indicated that the basic calibration equations were accurate for samples from different sources. Then, the verified sample sets were added to update the basic calibration. The SECV of the updated equations for CP, ADF and NDF were 0.81, 1.07 and 1.29%, the SEC were 0.64, 0.83 and 1.08% and the R^2 were 0.91, 0.92 and 0.93, respectively. The RPD (ratio of performance to deviation) were 3.4, 3.6 and 3.8 for CP, ADF and NDF, respectively. It indicated that the three calibration equations were acceptable for predicting the nutrient constituents accurately. The sample ranges of the calibration equations were diverse and the results demonstrated that the calibration equations established were accurate for constituents predicting. The calibration equations could be applied for components rapid analysis of forage corn and soybean intercropping.

並列關鍵字

Forage corn ； Soybean ； Near infrared spectroscopy ； Rapid analysis