蘭嶼豬內源性反轉錄病毒的檢測

Detection of porcine endogenous retroviruses in Lanyu pig

章嘉潔(Chia-Chieh Chang) ； 吳昇陽(Sheng-Yang Wu)

《畜產研究》 50卷2期 (2017/06) Pp. 103-109

摘要

本試驗旨在檢測蘭嶼豬豬內源性反轉錄病毒（porcine endogenous retrovirus, PERV）存在與表現狀況，採集飼養於臺東種畜繁殖場蘭嶼豬群38件的血液樣本，收集分離取得周邊血液單核球細胞（peripheral blood mononuclear cell, PBMC），利用PCR檢測PERV前病毒核心蛋白基因（gag）、多聚酶基因（pol）及囊膜基因（env）之DNA片段，另用RT-PCR方法檢測PERV前病毒mRNA序列片段。結果在38份DNA樣品中均檢測出了PERV前病毒組DNA的存在；另RT-PCR檢測38份RNA樣品中均有PERV前病毒RNA的表現。結論檢測蘭嶼豬體內均存在豬內源性反轉錄病毒，且能被轉錄以mRNA的形式表現，此結果提供蘭嶼豬建立PERV生物安全性評估之試驗基礎。

關鍵字

豬內源性反轉錄病毒 ； 蘭嶼豬 ； 聚合酶連鎖反應

並列摘要

This study was to investigate the existence and expression of porcine endogenous retrovirus (PERV) in Lanyu pig. The blood of 38 minipigs were collected from Taitung Animal Propagation Station. Peripheral blood mononuclear cell (PBMC) from blood samples were isolated. The existence of core protein gene (gag), polymerase gene (pol) and capsule membrane gene (env) from PERV provirus in DNA sequences were detected by PCR and expression of mRNA sequences of PERV provirus were tested by RT-PCR. The results showed that PERV provirus existed in all 38 DNA samples. Results of RT-PCR showed that PERV provirus expressed in 38 RNA samples. The PERV is existent and can be transcripted in Lanyu pig. This research may be helpful to assess the biosafety of PERV and to provide in experimental basis for Lanyu pigs.

並列關鍵字

Porcine endogenous retrovirus ； Lanyu pig ； Polymerase chain reaction