指草Survenola（Digitaria × umfolozi Hall）未成熟花穗培養與植株再生

Survenola（Digitaria × umfolozi Hall）為種間雜交的指草屬草種，染色體為6倍數，染色體數為54，主要以匍匐莖進行營養繁殖，為佛羅里達的熱帶草種育種計畫釋出的品種之一。本研究主要以Survenola的未成熟花穗為培植體，建立癒合組織誘導與植株再生的方法，期建立種原保存與種苗量化生產之技術。將Survenola的未成熟花穗經適當消毒後，培養於添加植物生長調節劑1.0、2.0 mg L^(-1) 2,4-D（2,4-dichlorophenoxyacetic acid）與0.0、0.1、0.5、1.0、2.0、4.0 mg L^(-1) BA（N6-benzyladenine）不同濃度組合之MS（Murashige and Skoog）培養基，探討2,4-D及BA對Survenola癒合組織誘導率及植株再生率之影響。試驗結果顯示，培養基添加2,4-D及BA對未成熟花穗癒合組織誘導率的主效應影響顯著，交感效應不顯著。其中2.0 mg L^(-1) 2,4-D癒合組織誘導率的主效應平均值為93.1%顯著高於1.0 mg L^(-1) 2,4-D的88.1%（P<0.05）。2,4-D與BA不同濃度組合誘導的癒合組織移至添加0.5 mg L^(-1) NAA（α-naphthaleneacetic acid）及0.1 mg L^(-1) TDZ（N-phenyl-N'-1,2,3-thiadiazol-5-yl urea）的植株分化培養基，2,4-D與BA對植株再生率並無顯著的影響。添加2 mg L^(-1) 2,4-D與1.0 mg L^(-1) BA誘導的癒合組織有60.0%可分化為植株，顯著高於其他處理組合（P<0.05）。根據本試驗建立之組織培養流程，將有助於Survenola種苗大量繁殖及未來應用生物技術協助指草屬牧草品種改良之研究。

關鍵字

指草；癒合組織；植株再生

並列摘要

Survenola (Digitaria × umfolozi Hall) is a hexaploid interspecific hybrid grass species with 6× = 54 chromosomes. Mainly vegetative propagated by stolons, Survenola is one of the varieties released from Florida's tropical grass breeding program. In this study, a tissue cultured method for callus induction and plant regeneration from the immature inflorescences of Survenola was established for variety preservation and mass production. In order to investigate the effects of 2,4-D and BA on the induction rate of Survenola callus and the rate of plant regeneration, the immature inflorescences of Survenola were properly sterilized and cultured with MS (Murashige and Skoog) medium and combined with different concentrations of plant growth regulators 1.0, 2.0 mg L^(-1) 2,4-D (2,4-dichlorophenoxyacetic acid) and 0.0, 0.1, 0.5, 1.0, 2.0, 4.0 mg L^(-1) BA (N6-benzyladenine).The experimental results showed that the main effect of medium supplementation with 2,4-D and BA on the induction rate of callus of immature inflorescences was significant, but the effect of interaction was not significant. In particular, the average figure of the main effect for 2.0 mg L^(-1) 2,4-D callus induction rate was 93.1%, which was significantly higher than 88.1% with 1.0 mg L^(-1) 2,4-D. The effects of 2,4-D and BA showed no significant effect on plant regeneration from the results of the callus induced by 2,4-D and BA were cultured on medium with 0.5 mg L^(-1) NAA (α-naphthaleneacetic acid) and 0.1 mg L^(-1) TDZ (N-phenyl-N'-1,2,3- thiadiazol-5-yl urea). There were 60% of callus induced with 2 mg L^(-1) 2,4-D and 1.0 mg L^(-1) BA that could be regenerated into plantlet, which was significantly higher than the combination of other treatments. The tissue culture process established according to this experiment will help the mass propagation of Survenola seedlings and the research on the improvement of forage varieties using biotechnology in the future.

並列關鍵字

Digitgrass ； Callus ； Plant regeneration

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