To promote innovation outcomes in Taiwan, government and research institute proactively launch a series of programs to develop advanced technologies to attract global investors. The government-led programs aim to initiate an international connection network, which is called regional entrepreneurial system-building. This mechanism not only includes individual invention or teams' innovation outcomes but also provides new startup ventures access to international funding. Therefore, we examine how to effectively build a regional entrepreneurial ecosystem in this study. Existing literature, however, pays too little attention to the role of the government and research institutes to discover the intrinsic values of these government-led programs. In addition, it is still unclear about the actual effects of these mechanisms. Consequently, we conduct a multiple-case study, collect secondary data, and study literature reviews to identify the key factors of the effective regional entrepreneurial ecosystem. Finally, we propose a conceptual framework for entrepreneurial management at the regional entrepreneurial ecosystem level.